Hippocampal Dendritic Spines Are Segregated Depending on Their Actin Polymerization
Domínguez Iturza, Nuria (Universitat Autònoma de Barcelona. Institut de Neurociències)
Calvo, María (Universitat de Barcelona)
Benoist, Marion (Aix-Marseille Université)
Esteban, José A (Centro de Biología Molecular Severo Ochoa)
Morales, Miguel (Universitat Autònoma de Barcelona. Institut de Neurociències)

Date:	2016
Abstract:	Dendritic spines are mushroom-shaped protrusions of the postsynaptic membrane. Spines receive the majority of glutamatergic synaptic inputs. Their morphology, dynamics, and density have been related to synaptic plasticity and learning. The main determinant of spine shape is filamentous actin. Using FRAP, we have reexamined the actin dynamics of individual spines from pyramidal hippocampal neurons, both in cultures and in hippocampal organotypic slices. Our results indicate that, in cultures, the actin mobile fraction is independently regulated at the individual spine level, and mobile fraction values do not correlate with either age or distance from the soma. The most significant factor regulating actin mobile fraction was the presence of astrocytes in the culture substrate. Spines from neurons growing in the virtual absence of astrocytes have a more stable actin cytoskeleton, while spines from neurons growing in close contact with astrocytes show a more dynamic cytoskeleton. According to their recovery time, spines were distributed into two populations with slower and faster recovery times, while spines from slice cultures were grouped into one population. Finally, employing fast lineal acquisition protocols, we confirmed the existence of loci with high polymerization rates within the spine.
Grants:	Ministerio de Economía y Competitividad BFU-2010-1753 Ministerio de Economía y Competitividad SAF-2011-24730
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Neural plasticity, Vol. 2016 (january 2016) , ISSN 1687-5443

DOI: 10.1155/2016/2819107
PMID: 26881098

13 p, 3.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Neurociències (INc)
Articles > Research articles
Articles > Published articles