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| Home > Articles > Published articles > Identification of genetic variation in the swine toll-like receptors and development of a porcine TLR genotyping array |
(Centre de Recerca en Agrigenòmica) (Jāmiʻat ʻAyn Shams) (Centre de Recerca en Agrigenòmica)| Date: | 2016 |
| Abstract: | Toll-like receptors (TLR) are crucial in innate immunity for the recognition of a broad range of microbial pathogens and are expressed in multiple cell types. There are 10 TLR genes described in the pig genome. With a twofold objective i. e. to catalogue genetic variants in porcine TLR genes and develop a genotyping array for genetic association studies on immune-related traits, we combined targeted sub-genome enrichment and high-throughput sequencing to sequence the 10 porcine TLR genes in 266 pigs from 10 breeds and wild boars using a DNA-pooling strategy. We identified 306 single nucleotide variants across the 10 TLR and 11 populations, 87 of which were novel. One hundred and forty-seven positions i. e. six stop-gains and 141 non-synonymous substitutions were predicted to alter the protein sequence. Three positions were unique to a single breed with alternative allele frequencies equal to or higher than 0. 5. We designed a genotyping array for future applications in genetic association studies, with a selection of 126 variants based on their predicted impact on protein sequence. Since TLR4, TLR7 and TLR9 were underrepresented in this selection, we also included three variants that were located in the 3'UTR of these genes. We tested the array by genotyping 214 of the 266 sequenced pigs. We found that 93 variants that involved the 10 TLR genes were polymorphic in these animals. Twelve of these variants were novel. Furthermore, seven known variants that are associated with immune-related phenotypes are present on the array and can thus be used to test such associations in additional populations. We identified genetic variations that potentially have an impact on the protein sequence of porcine TLR. A genotyping array with 80 non-synonymous, 10 synonymous and three 3'UTR polymorphisms in the 10 TLR genes is now available for association studies in swine populations with measures on immune-related traits. The online version of this article (doi:10. 1186/s12711-016-0206-0) contains supplementary material, which is available to authorized users. |
| Grants: | Ministerio de Economía y Competitividad AGL2013-44978-R Ministerio de Economía y Competitividad IPT-2012-0378-060000 Ministerio de Economía y Competitividad RYC-2011-07763 Ministerio de Ciencia e Innovación AGL2010-22358-C02-01 Ministerio de Ciencia e Innovación PTQ-12-05391 Ministerio de Economía y Competitividad CSD2007-00036 |
| Rights: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.  |
| Language: | Anglès |
| Document: | Article ; recerca ; Versió publicada |
| Published in: | Genetics, selection, evolution, Vol. 48 (2016) , art. 28, ISSN 1297-9686 |
