Inflammation affects the viability and plasticity of equine mesenchymal stem cells : possible implications in intra-articular treatments
Barrachina, Laura (Universidad de Zaragoza)
Remacha, Ana Rosa (Laboratory of Biochemical Genetics LAGENBIO, Veterinary Hospital, University of Zaragoza)
Romero, Antonio (Universidad de Zaragoza)
Vázquez, Francisco José (Universidad de Zaragoza)
Albareda, Jorge (Hospital Clínico Universitario "Lozano Blesa" de Zaragoza)
Prades, Marta (Universitat de Barcelona. Servei de Cirurgia Equina)
Ranera, Beatriz (Laboratory of Biochemical Genetics LAGENBIO, Veterinary Hospital, University of Zaragoza)
Zaragoza, Pilar (Laboratory of Biochemical Genetics LAGENBIO, Veterinary Hospital, University of Zaragoza)
Martín-Burriel, Inmaculada (Laboratory of Biochemical Genetics LAGENBIO, Veterinary Hospital, University of Zaragoza)
Rodellar, Clementina (Laboratory of Biochemical Genetics LAGENBIO, Veterinary Hospital, University of Zaragoza)

Date:	2017
Abstract:	Mesenchymal stem cells (MSCs) are gaining relevance for treating equine joint injuries because of their ability to limit inflammation and stimulate regeneration. Because inflammation activates MSC immunoregulatory function, proinflammatory priming could improve MSC efficacy. However, inflammatory molecules present in synovial fluid or added to the culture medium might have deleterious effects on MSCs. Therefore, this study was conducted to investigate the effects of inflammatory synovial fluid and proinflammatory cytokines priming on viability and plasticity of equine MSCs. Equine bone marrow derived MSCs (eBM-MSCs) from three animals were cultured for 72 h in media supplemented with: 20% inflammatory synovial fluid (SF); 50 ng/mL IFN-γ and TNF-α (CK50); and 20 ng/mL IFN-γ and TNF-α (CK20). Proliferation assay and expression of proliferation and apoptosis-related genes showed that SF exposed-eBM-MSCs maintained their viability, whereas the viability of CK primed-eBM-MSCs was significantly impaired. Tri-lineage differentiation assay revealed that exposure to inflammatory synovial fluid did not alter eBM-MSCs differentiation potential; however, eBM-MSCs primed with cytokines did not display osteogenic, adipogenic or chondrogenic phenotype. The inflammatory synovial environment is well tolerated by eBM-MSCs, whereas cytokine priming negatively affects the viability and differentiation abilities of eBM-MSCs, which might limit their in vivo efficacy.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Horses ; Mesenchymal stromal cells ; Joint diseases ; Proinflammatory cytokines ; Synovial fluid
Published in:	Journal of Veterinary Science, Vol. 18 (march 2017) , p. 39-49, ISSN 1976-555X

DOI: 10.4142/jvs.2017.18.1.39
PMID: 27297420

11 p, 6.3 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
Articles > Research articles
Articles > Published articles