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Genome sequence of the olive tree, Olea europaea
Cruz, Fernando (Universitat Pompeu Fabra)
Julca, Irene (Universitat Autònoma de Barcelona)
Gómez-Garrido, Jèssica (Universitat Pompeu Fabra)
Loska, Damian (The Barcelona Institute of Science and Technology)
Marcet-Houben, Marina (The Barcelona Institute of Science and Technology)
Cano, Emilio (Consejo Superior de Investigaciones Científicas)
Galán, Beatriz (Consejo Superior de Investigaciones Científicas)
Frias, Leonor (Universitat Pompeu Fabra)
Ribeca, Paolo (Universitat Pompeu Fabra)
Derdak, Sophia (Universitat Pompeu Fabra)
Gut, Marta (Universitat Pompeu Fabra)
Sánchez Fernández, Manuel (Grupo Santander)
García, José Luis (Consejo Superior de Investigaciones Científicas)
Gut, Ivo (Universitat Pompeu Fabra)
Vargas, Pablo (Vargas Gómez) (Royal Botanical Garden of Madrid)
Alioto, Tyler (Centre Nacional d'Anàlisi Genòmica)
Gabaldón, Toni (CRG-Centre for Genomic Regulation)

Date: 2016
Abstract: The Mediterranean olive tree (Olea europaea subsp. europaea) was one of the first trees to be domesticated and is currently of major agricultural importance in the Mediterranean region as the source of olive oil. The molecular bases underlying the phenotypic differences among domesticated cultivars, or between domesticated olive trees and their wild relatives, remain poorly understood. Both wild and cultivated olive trees have 46 chromosomes (2n). A total of 543 Gb of raw DNA sequence from whole genome shotgun sequencing, and a fosmid library containing 155,000 clones from a 1,000+ year-old olive tree (cv. Farga) were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome with a scaffold N50 of 443 kb, and a total length of 1. 31 Gb, which represents 95 % of the estimated genome length (1. 38 Gb). In addition, the associated fungus Aureobasidium pullulans was partially sequenced. Genome annotation, assisted by RNA sequencing from leaf, root, and fruit tissues at various stages, resulted in 56,349 unique protein coding genes, suggesting recent genomic expansion. Genome completeness, as estimated using the CEGMA pipeline, reached 98. 79 %. The assembled draft genome of O. europaea will provide a valuable resource for the study of the evolution and domestication processes of this important tree, and allow determination of the genetic bases of key phenotypic traits. Moreover, it will enhance breeding programs and the formation of new varieties.
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Olive tree genome ; Genomics ; Assembly ; Annotation
Published in: GigaScience, Vol. 5, Issue 1 (December 2016) , art. 29, ISSN 2047-217X

PMID: 27346392
DOI: 10.1186/s13742-016-0134-5


12 p, 1.0 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2018-02-07, last modified 2019-10-07



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