peIF4E as an independent prognostic factor and a potential therapeutic target in diffuse infiltrating astrocytomas
Martínez Sáez, Elena (Hospital Universitari Vall d'Hebron)
Peg, Vicente (Hospital Universitari Vall d'Hebron)
Ortega Aznar, Arantxa (Hospital Universitari Vall d'Hebron)
Martínez Ricarte, Francisco (Hospital Universitari Vall d'Hebron)
Camacho, Jessica (Hospital Universitari Vall d'Hebron)
Hernández Losa, Javier (Hospital Universitari Vall d'Hebron)
Ferreres Piñas, Joan Carles (Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí (I3PT))
Ramón y Cajal, Santiago (Hospital Universitari Vall d'Hebron)
Universitat Autònoma de Barcelona. Departament de Ciències Morfològiques

Fecha:	2016
Resumen:	Malignant transformation in tumors is a complex process requiring accumulation of numerous oncogenic abnormalities. Brain tumors show considerable phenotypic and genetic heterogeneity. In a series comprising diffuse infiltrating astrocytomas () and reactive gliosis, we investigated the main factors associated with signaling pathways. We assessed expression levels and their association with tumor progression and survival. We studied 19 grade astrocytomas, 25 anaplastic astrocytomas (grade ), 60 glioblastomas (grade ), and 15 cases of reactive gliosis. Epidermal growth factor receptor (), , 4E-1, p4E-1, 6, 4E, and pe4E expression levels were evaluated using immunohistochemistry. Expression levels were semiquantitatively evaluated using a histoscore. Immunohistochemistry and were used for 1 mutations. Statistical analysis was based on the following tests: chi-square, Student's t, Pearson correlation, Spearman's rho, and Mann-Whitney; and Kaplan-Meier curves were constructed. A significant increase was observed between grades for expression of total and phosphorylated 4E-1 and for 4E, Ki67, , and cyclin D1. Although expression of ,4E, and Ki67 correlated with survival, only pe4E was an independent predictor of survival in the multivariate analysis. Combining the evaluation of different proteins enables us to generate helpful diagnostic nomograms. In conclusion, cell signaling pathways are activated in s; pe4E is an independent prognostic factor and a promising therapeutic target. Joint analysis of the expression of 4E-1 and pe4E could be helpful in the diagnosis of glioblastoma multiforme in small biopsy samples.
Ayudas:	Instituto de Salud Carlos III PI11/00185 Instituto de Salud Carlos III PI08/0143 Instituto de Salud Carlos III RD12/0036/0057 Instituto de Salud Carlos III CM09/00143 Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-756
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Astrocytomas ; Cell signaling ; Glioblastoma ; Pelf4e ; Prognosis
Publicado en:	Cancer Medicine, Vol. 5, issue 9 (Sep. 2016) , p. 2501-2512, ISSN 2045-7634

DOI: 10.1002/cam4.817
PMID: 27440383

12 p, 1.1 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Instituto de Investigación e Innovación Parc Taulí (I3PT)
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