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Collaborative Evaluation of Optimal Antifungal Susceptibility Testing Conditions for Dermatophytes
Fernández-Torres, Belkys (Universitat Rovira i Virgili. Unitat de Microbiologia)
Cabañes Sáenz, Francisco Javier (Universitat Autònoma de Barcelona Departament de Sanitat i d'Anatomia Animals)
Carrillo-Muñoz, Alfonso J. (Asesoría Científica y de Investigación Aplicada)
Esteban, Alexandre (Universitat Autònoma de Barcelona Departament de Sanitat i d'Anatomia Animals)
Inza, Isabel (Universitat Rovira i Virgili. Unitat de Microbiologia)
Abarca Salat, Ma. Lourdes (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Guarro Artigas, Josep (Universitat de Barcelona. Càtedra de Microbiologia)

Date: 2002
Abstract: A multicenter study was conducted to define the most suitable testing conditions for antifungal susceptibility of dermatophytes. Broth microdilution MICs of clotrimazole, itraconazole, and terbinafine were determined in three centers against 60 strains of dermatophytes. The effects of inoculum density (ca. 103 and 104 CFU/ml), incubation time (3, 7, and 14 days), endpoint criteria for MIC determination (complete [MIC-0] and prominent [MIC-2] growth inhibition), and incubation temperature (28 and 37°C) on intra- and interlaboratory agreement were analyzed. The optimal testing conditions identified were an inoculum of 104 CFU/ml, a temperature of incubation of 28°C, an incubation period of 7 days, and MIC-0.
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Language: Anglès
Document: article ; recerca ; publishedVersion
Subject: Micologia Veterinària
Published in: Journal of clinical microbiology, Vol. 40 Núm. 11 (Novembre 2002) , p. 3999-4003, ISSN 0095-1137

DOI: 10.1128/jcm.40.11.3999-4003.2002
PMID: 12409365

5 p, 66.8 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Veterinary Mycology Group
Articles > Research articles
Articles > Published articles

 Record created 2018-06-14, last modified 2020-12-07

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