Detection of Bacteriophage Particles Containing Antibiotic Resistance Genes in the Sputum of Cystic Fibrosis Patients
Brown-Jaque, Maryury (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Rodriguez Oyarzun, Lirain (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Cornejo-Sánchez, Thais (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Martín-Gómez, Maria T. (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Gartner, Silvia (Hospital Universitari Vall d'Hebron)
De Gracia, Javier (Hospital Universitari Vall d'Hebron)
Rovira, Sandra (Hospital Universitari Vall d'Hebron)
Álvarez Fernández, Antonio (Hospital Universitari Vall d'Hebron)
Jofre, Joan (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
González-López, Juanjo 1975- (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Muniesa, Maite (Universitat de Barcelona. Departament de Genètica, Microbiologia i Estadística)
Universitat Autònoma de Barcelona

Data:	2018
Resum:	Cystic fibrosis (CF) is a chronic disease in which the bacterial colonization of the lung is linked to an excessive inflammatory response that leads to respiratory failure. The microbiology of CF is complex. Staphylococcus aureus is the first bacterium to colonize the lungs in 30% of pediatric CF patients, and 80% of adult patients develop a chronic Pseudomonas aeruginosa infection, but other microorganisms can also be found. The use of antibiotics is essential to treat the disease, but antibiotic performance is compromised by resistance mechanisms. Among various mechanisms of transfer of antibiotic resistance genes (ARGs), the recently been reported bacteriophages are the least explored in clinical settings. To determine the role of phages in CF as mobile genetic elements (MGEs) carrying ARGs, we evaluated their presence in 71 CF patients. 71 sputum samples taken from these patients were screened for eight ARGs (bla , bla -group, bla -group, bla , bla , mecA, qnrA, and qnrS) in the bacteriophage DNA fraction. The phages found were also purified and observed by electron microscopy. 32. 4% of CF patients harbored ARGs in phage DNA. β-lactamase genes, particularly bla and bla , were the most prevalent and abundant, whereas mecA, qnrA, and qnrS were very rare. Siphoviridae phage particles capable of infecting P. aeruginosa and Klebsiella pneumoniae were detected in CF sputum. Phage particles harboring ARGs were found to be abundant in the lungs of both CF patients and healthy individuals and could contribute to the colonization of multiresistant strains.
Ajuts:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2009SGR1043
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Cystic fibrosis ; Bacteriophages ; Antibiotic resistance genes ; Horizontal gene transfer ; Sputum
Publicat a:	Frontiers in microbiology, Vol. 9 (may 2018) , ISSN 1664-302X

DOI: 10.3389/fmicb.2018.00856
PMID: 29765367

10 p, 1.6 MB

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