Production of biofunctionalized MoS2 flakes with rationally modified lysozyme : A biocompatible 2D hybrid material
Siepi, Maria Luisa (Institut Català de Nanociència i Nanotecnologia)
Morales-Narváez, Eden (Institut Català de Nanociència i Nanotecnologia)
Domingo Marimon, Neus (Institut Català de Nanociència i Nanotecnologia)
Monti, Daria Maria (Università degli studi di Napoli Federico II)
Notomista, Eugenio (Università degli studi di Napoli Federico II)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date:	2017
Abstract:	Bioapplications of 2D materials embrace demanding features in terms of environmental impact, toxicity and biocompatibility. Here we report on the use of a rationally modified lysozyme to assist the exfoliation of Mos bulk crystals suspended in water through ultrasonic exfoliation. The design of the proposed lysozyme derivative provides this exfoliated 2D-materail with both, hydrophobic groups that interact with the surface of Mos and hydrophilic groups exposed to the aqueous medium, which hinders its re-Aggregation. This approach, clarified also by molecular docking studies, leads to a stable material (ζ-potential, 27 ?} 1 mV) with a yield of up to 430 μg ml-1. The bio-hybrid material was characterized in terms of number of layers and optical properties according to different slots separated by diverse centrifugal forces. Furthermore the obtained material was proved to be biocompatible using human normal keratinocytes and human cancer epithelial cells, whereas the method was demonstrated to be applicable to produce other 2D materials such as graphene. This approach is appealing for the advantageous production of high quality Mos flakes and their application in biomedicine and biosensing. Moreover, this method can be applied to different starting materials, making the denatured lysozyme a promising bio-Tool for surface functionalization of 2D materials.
Grants:	Ministerio de Economía y Competitividad SEV-2013-0295 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-260
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	2D materials ; Biocompatibility ; Biofunctionalized material
Published in:	2D materials, Vol. 4, Núm. 3 (September 2017) , article 035007, ISSN 2053-1583

DOI: 10.1088/2053-1583/aa7966

Post-print 19 p, 996.4 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles