The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides
Sun, Xiaoming (Chinese Academy of Sciences)
Matus, José Tomás (Centre de Recerca en Agrigenòmica)
Wong, Darren Chern Jan (Australian National University. Research School of Biology. Ecology and Evolution)
Wang, Zemin (Chinese Academy of Sciences)
Chai, Fengmei (Chinese Academy of Sciences. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture)
Zhang, Langlang (Chinese Academy of Sciences. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture)
Fang, Ting Ting (Chinese Academy of Sciences. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture)
Zhao, Li (Chinese Academy of Sciences. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture)
Wang, Yi (Chinese Academy of Sciences)
Han, Yuepeng (Chinese Academy of Sciences. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture)
Wang, Qingfeng (Chinese Academy of Sciences. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture)
Li, Shaohua (Chinese Academy of Sciences)
Liang, Zhenchang (Chinese Academy of Sciences)
Xin, Haiping (Chinese Academy of Sciences. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture)

Date:	2018
Abstract:	Grapevine (Vitis vinifera L. ) is a widely cultivated fruit crop whose growth and productivity are greatly affected by low temperatures. On the other hand, wild Vitis species represent valuable genetic resources of natural stress tolerance. We have isolated and characterized a MYB-like gene encoding a putative GARP-type transcription factor from Amur grape (V. amurensis) designated as VaAQUILO. AQUILO (AQ) is induced by cold in both V. amurensis and V. vinifera, and its overexpression results in significantly improved tolerance to cold both in transgenic Arabidopsis and in Amur grape calli. In Arabidopsis, the ectopic expression of VaAQ increased antioxidant enzyme activities and up-regulated reactive oxygen species- (ROS) scavenging-related genes. Comparative mRNA sequencing profiling of 35S:VaAQ Arabidopsis plants suggests that this transcription factor is related to phosphate homeostasis like their Arabidopsis closest homologues: AtHRS1 and AtHHO2. However, when a cold stress is imposed, AQ is tightly associated with the cold-responsive pathway and with the raffinose family oligosaccharides (RFOs), as observed by the up-regulation of galactinol synthase (GoLS) and raffinose synthase genes. Gene co-expression network (GCN) and cis-regulatory element (CRE) analyses in grapevine indicated AQ as potentially regulating VvGoLS genes. Increased RFO content was confirmed in both transgenic Arabidopsis and Amur grape calli overexpressing VaAQ. Taken together, our results imply that AQ improves cold tolerance through promoting the accumulation of osmoprotectants.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Abiotic stress ; Chinese wild grape ; Cold response ; Galactinol synthase ; Grape transformation ; HRS1 ; Low temperature exotherms ; Raffinose ; ROS
Published in:	Journal of experimental botany, Vol. 69 Núm. 7 (March 2018) , p. 1749-1764, ISSN 1460-2431

DOI: 10.1093/jxb/ery020
PMID: 29385617

16 p, 18.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
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