Web of Science: 9 cites, Scopus: 11 cites, Google Scholar: cites,
Inflammatory conditions dictate the effect of Mesenchymal stem or Stromal cells on B cell function.
Luk, Franka (Erasmus University Medical Center, Rotterdam)
Carreras-Planella, Laura (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Korevaar, Sander S. (Erasmus University Medical Center, Rotterdam)
de Witte, Samantha F. H. (Erasmus University Medical Center, Rotterdam)
Borràs, Francesc E. (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, Fisiologia i Immunologia)
Betjes, Michiel G. H. (Erasmus University Medical Center, Rotterdam)
Baan, Carla C. (Erasmus University Medical Center, Rotterdam)
Hoogduijn, Martin J. (Erasmus University Medical Center, Rotterdam)
Franquesa, Marcella (Erasmus University Medical Center, Rotterdam)

Data: 2017
Resum: The immunomodulatory capacity of mesenchymal stem or stromal cells (MSC) makes them a promising tool for treatment of immune disease and organ transplantation. The effects of MSC on B cells are characterized by an abrogation of plasmablast formation and induction of regulatory B cells (Bregs). It is, however, unknown how MSC interact with B cells under inflammatory conditions. In this study, adipose tissue-derived MSC were pretreated with 50 ng/ml IFN-γ for 96 h (MSC–IFN-γ) to simulate inflammatory conditions. Mature B cells were obtained from spleens by CD43− selection. B cells were co-cultured with MSC and stimulated with anti-IgM, anti-CD40, and IL-2; and after 7 days, B cell proliferation, phenotype, Immunoglobulin-G (IgG), and IL-10 production were analyzed. MSC did not inhibit B cell proliferation but increased the percentage of CD38high CD24high B cells (Bregs) and IL-10 production, while MSC–IFN-γ significantly reduced B cell proliferation and inhibited IgG production by B cells in a more potent fashion but did not induce Bregs or IL-10 production. Both MSC and MSC–IFN-γ required proximity to target cells and being metabolically active to exert their effects. Indoleamine 2,3 dioxygenase expression was highly induced in MSC–IFN-γ and was responsible of the anti-proliferative and Breg reduction since addition of tryptophan (TRP) restored MSC properties. Immunological conditions dictate the effect of MSC on B cell function. Under immunological quiescent conditions, MSC stimulate Breg induction; whereas, under inflammatory conditions, MSC inhibit B cell proliferation and maturation through depletion of TRP. This knowledge is useful for customizing MSC therapy for specific purposes by appropriate pretreatment of MSC.
Nota: Número d'acord de subvenció AGAUR/2014BP B00118
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès.
Document: article ; recerca ; publishedVersion
Matèria: B cell ; Immunomodulation ; Mesenchymal stem cell ; Plasmablast ; Regulatory B cell ; Indoleamine ; 2,3-dioxygenase
Publicat a: Frontiers in immunology, Vol. 8 (august 2017) , p. 1-13

DOI: 10.3389/fimmu.2017.01042
PMID: 28894451


13 p, 6.6 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Articles de recerca
Articles > Articles publicats

 Registre creat el 2018-10-24, darrera modificació el 2019-05-27



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