An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection
Mayorga Martinez, Carmen (Institut Català de Nanociència i Nanotecnologia)
Chamorro García, Alejandro (Institut Català de Nanociència i Nanotecnologia)
Serrano, Lorena (Vetgenomics)
Rivas, Lourdes (Institut Català de Nanociència i Nanotecnologia)
Quesada-González, Daniel (Institut Català de Nanociència i Nanotecnologia)
Altet Sanahujes, Laura (Vetgenomics)
Francino, Olga (Vetgenomics)
Sánchez Bonastre, Armando (Vetgenomics)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date:	2015
Abstract:	An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO₂ NP/). An aminated oligonucleotide probe is immobilized on the IrO₂ NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.
Grants:	European Commission 315653 Ministerio de Economía y Competitividad SEV-2013-0295 Ministerio de Economía y Competitividad MAT2011-25870
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Blocking agents ; DNA detection ; Genosensors ; Iridium oxides ; Modified electrodes ; Oligonucleotide probes ; Reproducibilities ; Screen-printed carbon electrodes
Published in:	Journal of materials chemistry B, Vol. 3, issue 26 (July 2015) , p. 5166-5171, ISSN 2050-7518

DOI: 10.1039/c5tb00545k

Postprint 14 p, 732.2 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles