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An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection
Mayorga Martinez, Carmen (Institut Catala de Nanociencia i Nanotecnologia)
Chamorro García, Alejandro (Institut Català de Nanociència i Nanotecnologia)
Serrano, Lorena (Vetgenomics)
Rivas, Lourdes (Institut Català de Nanociència i Nanotecnologia)
Quesada González, Daniel (Institut Català de Nanociència i Nanotecnologia)
Altet, Laura (Vetgenomics)
Francino, Olga (Vetgenomics)
Sánchez Bonastre, Armando (Vetgenomics)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date: 2015
Abstract: An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO₂ NP/). An aminated oligonucleotide probe is immobilized on the IrO₂ NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.
Note: Número d'acord de subvenció EC/FP7/315653
Note: Número d'acord de subvenció MINECO/SEV-2013-0295
Note: Número d'acord de subvenció MINECO/MAT2011-25870
Rights: Tots els drets reservats.
Language: Anglès
Document: article ; recerca ; acceptedVersion
Subject: Blocking agents ; DNA detection ; Genosensors ; Iridium oxides ; Modified electrodes ; Oligonucleotide probes ; Reproducibilities ; Screen-printed carbon electrodes
Published in: Journal of materials chemistry B, Vol. 3, issue 26 (July 2015) , p. 5166-5171, ISSN 2050-7518

DOI: 10.1039/c5tb00545k

14 p, 732.2 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles

 Record created 2018-12-17, last modified 2020-11-09

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