Annexin-V/quantum dot probes for multimodal apoptosis monitoring in living cells : Improving bioanalysis using electrochemistry
Montón i Domingo, Helena (Institut Català de Nanociència i Nanotecnologia)
Parolo, Claudio (Institut Català de Nanociència i Nanotecnologia)
Aranda-Ramos, Antonio (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)
Nogués, C. (Carme) (Carme) (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Date:	2015
Abstract:	There is a great demand to develop novel techniques that allow useful and complete monitoring of apoptosis, which is a key factor of several diseases and a target for drug development. Here, we present the use of a novel dual electrochemical/optical label for the detection and study of apoptosis. We combined the specificity of Annexin-V for phosphatidylserine, a phospholipid expressed in the outer membrane of apoptotic cells, with the optical and electrochemical properties of quantum dots to create a more efficient label. Using this conjugate we addressed three important issues: (i) we made the labeling of apoptotic cells faster (30 min) and easier; (ii) we fully characterized the samples by common cell biological techniques (confocal laser scanning microscopy, scanning electron microscopy and flow cytometry); and (III) we developed a fast, cheap and quantitative electrochemical detection method for apoptotic cells with results in full agreement with those obtained by flow cytometry.
Grants:	European Commission 613844 Ministerio de Ciencia e Innovación MAT2011-25870 Ministerio de Ciencia e Innovación TEC2011-29140-C03-03 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-524
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Apoptotic cells ; Biological techniques ; Confocal laser scanning microscopy ; Drug development ; Electrochemical detection method ; Novel techniques ; Outer membrane ; Phosphatidylserine ; Annexin A5 ; Apoptosis ; Cell Line ; Dimethyl Sulfoxide ; Electrochemical Techniques ; Flow Cytometry ; Humans ; Microscopy, Confocal ; Microscopy, Electron, Scanning ; Phosphatidylserines ; Protein Binding ; Quantum Dots ; Surface Properties
Published in:	Nanoscale, Vol. 7, Núm. 9 (March 2015) , p. 4097-4104, ISSN 2040-3372

DOI: 10.1039/c4nr07191c

Postprint 12 p, 3.7 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles