Protection of Spanish Ibex (Capra pyrenaica) against Bluetongue Virus Serotypes 1 and 8 in a Subclinical Experimental Infection
Lorca Oró, Cristina (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Pujols, Joan (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
García-Bocanegra, Ignacio (Universitat Autònoma de Barcelona. Servei d'Ecopatologia de Fauna Salvatge)
Mentaberre García, Gregorio (Universitat Autònoma de Barcelona. Servei d'Ecopatologia de Fauna Salvatge)
Granados Torres, José Enrique (Espacio Natural Sierra Nevada)
Solanes Foz, David (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Fandos, Paulino (Parque Natural Sierras de Cazorla, Segura y Las Villas)
Galindo Cardiel, Iván (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Domingo, Mariano (Universitat Autònoma de Barcelona. Departament de Patologia i de Producció Animals)
Lavín González, Santiago (Universitat Autònoma de Barcelona. Servei d'Ecopatologia de Fauna Salvatge)
López Olvera, Jorge R. (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Date:	2012
Abstract:	Many wild ruminants such as Spanish ibex (Capra pyrenaica) are susceptible to Bluetongue virus (BTV) infection, which causes disease mainly in domestic sheep and cattle. Outbreaks involving either BTV serotypes 1 (BTV-1) and 8 (BTV-8) are currently challenging Europe. Inclusion of wildlife vaccination among BTV control measures should be considered in certain species. In the present study, four out of fifteen seronegative Spanish ibexes were immunized with a single dose of inactivated vaccine against BTV-1, four against BTV-8 and seven ibexes were non vaccinated controls. Seven ibexes (four vaccinated and three controls) were inoculated with each BTV serotype. Antibody and IFN-gamma responses were evaluated until 28 days after inoculation (dpi). The vaccinated ibexes showed significant (P,0. 05) neutralizing antibody levels after vaccination compared to non vaccinated ibexes. The non vaccinated ibexes remained seronegative until challenge and showed neutralizing antibodies from 7 dpi. BTV RNA was detected in the blood of non vaccinated ibexes from 2 to the end of the study (28 dpi) and in target tissue samples obtained at necropsy (8 and 28 dpi). BTV-1 was successfully isolated on cell culture from blood and target tissues of non vaccinated ibexes. Clinical signs were unapparent and no gross lesions were found at necropsy. Our results show for the first time that Spanish ibex is susceptible and asymptomatic to BTV infection and also that a single dose of vaccine prevents viraemia against BTV-1 and BTV-8 replication.
Note:	Altres ajuts: INIA/FAU2008-00019-C03-01
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Català
Document:	Article ; recerca ; Versió publicada
Subject:	Veterinària ; Llengua blava ; Ovelles ; Virosi
Published in:	PloS one, Vol. 7 Núm. 5 (2012) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0036380
PMID: 22666321

9 p, 1.3 MB

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