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Página principal > Artículos > Artículos publicados > Microencapsulation with alginate/CaCO 3 : |
Fecha: | 2017 |
Resumen: | Bacteriophages are promising therapeutic agents that can be applied to different stages of the commercial food chain. In this sense, bacteriophages can be orally administered to farm animals to protect them against intestinal pathogens. However, the low pH of the stomach, the activities of bile and intestinal tract enzymes limit the efficacy of the phages. This study demonstrates the utility of an alginate/CaCO encapsulation method suitable for bacteriophages with different morphologies and to yield encapsulation efficacies of ∼100%. For the first time, a cocktail of three alginate/CaCO-encapsulated bacteriophages was administered as oral therapy to commercial broilers infected with Salmonella under farm-like conditions. Encapsulation protects the bacteriophages against their destruction by the gastric juice. Phage release from capsules incubated in simulated intestinal fluid was also demonstrated, whereas encapsulation ensured sufficient intestinal retention of the phages. Moreover, the small size of the capsules (125-150 μm) enables their use in oral therapy and other applications in phage therapy. This study evidenced that a cocktail of the three alginate/CaCO-encapsulated bacteriophages had a greater and more durable efficacy than a cocktail of the corresponding non-encapsulated phages in as therapy in broilers against Salmonella, one of the most common foodborne pathogen. |
Ayudas: | Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-572 Agència de Gestió d'Ajuts Universitaris i de Recerca 2010/VALOR00114 Ministerio de Economía y Competitividad BIO2016-77011-R Ministerio de Economía y Competitividad SEV-2013-0295 |
Nota: | Altres ajuts: La Caixa and the Associació Catalana d'Universitats Públiques 2010ACUP00300 |
Derechos: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió publicada |
Materia: | Alginates ; Animals ; Body Fluids ; Calcium Carbonate ; Cecum ; Chickens ; Drug Compounding ; Glucuronic Acid ; Hexuronic Acids ; Imaging, Three-Dimensional ; Phage Therapy ; Salmonella ; Salmonella Infections, Animal |
Publicado en: | Scientific reports, Vol. 7 (January 2017) , art. 41441, ISSN 2045-2322 |
10 p, 1004.6 KB |