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ASPN and GJB2 are implicated in the mechanisms of invasion of ductal breast carcinomas
Castellana, Bàrbara (Institut d'Investigació Biomèdica Sant Pau)
Escuin, Daniel (Institut d'Investigació Biomèdica Sant Pau)
Peiró, Gloria (Hospital General Universitario de Alicante)
Garcia Valdecasas, Bárbara (Universitat Autònoma de Barcelona)
Vázquez, Tania (Institut d'Investigació Biomèdica Sant Pau)
Pons, Cristina (Institut d'Investigació Biomèdica Sant Pau)
Pérez Olabarria, Maitane (Institut d'Investigació Biomèdica Sant Pau)
Barnadas i Molins, Agustí (Universitat Autònoma de Barcelona. Departament de Medicina)
Lerma Puertas, Enrique (Universitat Autònoma de Barcelona. Departament de Ciències Morfològiques)

Date: 2012
Abstract: The mechanism of progression from ductal carcinoma in situ (DCIS) to invasive ductal carcinoma (IDC) remains largely unknown. We compared gene expression in tumors with simultaneous DCIS and IDC to decipher how diverse proteins participate in the local invasive process. Twenty frozen tumor specimens with concurrent, but separated, DCIS and IDC were mi-crodissected and evaluated. Total RNA was extracted and microarray analysis was performed using Affymetrix GeneChip® Human Gene 1. 0 ST Arrays. Microarray data were validated by quantitative real time reverse transcription-PCR (qRT-PCR) and immunohistochemistry. Controls included seven pure in situ carcinomas, eight fragments from normal breast tissue, and a series of mouse breast carcinomas (MMTV-PyMT). Fifty-six genes were differentially expressed between DCIS and IDC samples. The genes upregulated in IDC samples, and probably associated with invasion, were related to the ep-ithelial-mesenchymal transition (ASPN, THBS2, FN1, SPARC, and COL11A1), cellular adhesion (GJB2), cell motility and progression (PLAUR, PLAU, BGN, ADAMTS16, and ENPP2), extracellular matrix degradation (MMP11, MMP13, and MMP14), and growth/proliferation (ST6GAL2). qRT-PCR confirmed the expression patterns of ASPN, GJB2, ENPP2, ST6GAL2, and TMBS10. Expression of the ASPN and GJB2 gene products was detected by immunohistochemistry in invasive carcinoma foci. The association of GJB2 protein expression with invasion was con-firmed by qRT-PCR in mouse tumors (P < 0. 05). Conclusions: The upregulation of ASPN and GJB2 may play important roles in local invasion of breast ductal carcinomas.
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: ASPN ; Breast cancer ; Epithelial-mesenchymal transition ; GJB2 ; In situ ductal carcinoma ; Invasive ductal carcinoma
Published in: Journal of Cancer, Vol. 3, Núm. 1 (2012) , p. 175-183, ISSN 1837-9664

Adreça alternativa: http://www.jcancer.org/v03p0175.htm
DOI: 10.7150/jca.4120
PMID: 22514560

9 p, 652.1 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut d'Investigació Biomèdica Sant Pau
Articles > Research articles
Articles > Published articles

 Record created 2019-05-27, last modified 2019-08-28

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