Web of Science: 1 citations, Scopus: 1 citations, Google Scholar: citations,
Fully printed one-step biosensing device using graphene/AuNPs composite
Nagar, Bhawna (Institut Català de Nanociència i Nanotecnologia)
Balsells, Marc (Institut Català de Nanociència i Nanotecnologia)
De la Escosura Muñiz, Alfredo (Institut Català de Nanociència i Nanotecnologia)
Gómez-Romero, Pedro (Institut Català de Nanociència i Nanotecnologia)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date: 2019
Abstract: Driven by the growing need of simple, cost efficient and flexible sensing systems, we have designed here a fully printed Reduced Graphene Oxide (rGO) based impedimetric sensor for one step sensing of DNA. The DNA sensor was fabricated by stamping of layered rGO and rGO/gold nanoparticles/single stranded DNA (rGO/AuNPs/ssDNA) composites over PET substrates using wax-printing technique. rGO works as an excellent working electrode, while the AuNPs create a suitable environment for ssDNA immobilization. Counter and reference electrodes were previously screen-printed on the plastic substrate, making thus a compact and highly integrated sensing platform. The change in electron transfer resistance after hybridization with a target ssDNA specific of Coxsackie B3 virus was monitored using electrochemical impedance spectroscopy (EIS), finding a linear response in the range of concentrations 0. 01–20 µM. The novel, simple and straightforward one-step printing process for fabrication of a biosensing device developed keeps in mind the growing need of large scale device manufacturing. The successful proof-of-concept for the detection of DNA hybridization can be extended to other affinity biosensors, taking advantage of the integration of the bioreceptor on the sensor surface. Such ready-to-use biosensor would lead to a one-step electrochemical detection.
Note: Número d'acord de subvenció MINECO/MAT2017-87202-P
Note: Número d'acord de subvenció MINECO/SEV-2013-0295
Note: Número d'acord de subvenció EC/H2020/785219
Rights: Tots els drets reservats.
Language: Anglès.
Document: article ; recerca ; submittedVersion
Subject: RGO ; Wax stamping ; Screen printing ; Electrochemistry ; DNA ; Biosensing
Published in: Biosensors and bioelectronics, Vol. 129 (March 2019) , p. 238-244, ISSN 1873-4235

DOI: 10.1016/j.bios.2018.09.073


Preprint
24 p, 1.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles

 Record created 2019-07-02, last modified 2019-07-26



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