Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis
Gordillo-Marroquín, Cristina (Global Alliance for Rapid Diagnostics. Michigan State University)
Gómez-Velasco, Anaximandro (Global Alliance for Rapid Diagnostics. Michigan State University)
Sánchez-Pérez, Héctor J. (Global Alliance for Rapid Diagnostics. Michigan State University)
Pryg, Kasey (Nano-Biosensors Laboratory, Department of Biosystems and Agricultural Engineering, Michigan State University)
Shinners, John (Nano-Biosensors Laboratory, Department of Biosystems and Agricultural Engineering, Michigan State University)
Murray, Nathan (Nano-Biosensors Laboratory, Department of Biosystems and Agricultural Engineering, Michigan State University)
Muñoz-Jiménez, Sergio G. (Mycobacteriology Laboratory, TB Prevention and Control Program for the Highlands of Chiapas, Mexico)
Bencomo-Alerm, Allied (Mycobacteriology Laboratory, TB Prevention and Control Program for the Highlands of Chiapas, Mexico)
Gómez-Bustamante, Adriana (State Public Health Laboratory for Chiapas, Tuxtla Gutierrez, Chiapas Mexico)
Jonapá-Gómez, Letisia (State Public Health Laboratory for Chiapas, Tuxtla Gutierrez, Chiapas, Mexico)
Enríquez-Ríos, Natán (Ministerio de Sanidad de Cihiapas (Mèxic))
Martín Mateo, Miguel (Universitat Autònoma de Barcelona. Departament de Pediatria, Obstetrícia i Ginecologia i de Medicina Preventiva i Salut Pública)
Romero-Sandoval, Natalia (Faculty of Medical Sciences, and Health and Life, International University of Ecuador, Quito)
Alocilja, Evangelyn C. (Nano-Biosensors Laboratory, Department of Biosystems and Agricultural Engineering, Michigan State University)

Data:	2018
Resum:	A new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patients prior to receiving treatment. Experiments were also conducted to determine the appropriate concentration of glycan-functionalized magnetic nanoparticles (GMNP) used in the NCBA and to evaluate the optimal digestion/decontamination solution to increase the extraction, concentration and detection of acid-fast bacilli (AFB). The optimized NCBA consisted of a 1:1 mixture of 0. 4% NaOH and 4% N-acetyl-L-cysteine (NALC) to homogenize the sputum sample. Additionally, 10 mg/mL of GMNP was added to isolate and concentrate the AFB. All TB positive sputum samples were identified with an increased AFB count of 47% compared to SSM, demonstrating GMNP's ability to extract and concentrate AFB. Results showed that NCBA increased AFB count compared to SSM, improving the grade from "1+" (in SSM) to "2+". Extending the finding to paucibacillary cases, there is the likelihood of a "scant" grade to become "1+". The assay uses a simple magnet and only costs.
Nota:	Ajuts: This research was funded by the W.K. Kellogg Foundation, Programa de Investigación Integradode Tuberculosis PII-TB-SEPAR (grant number 058-2015), and by the Mexican National Council for Science andTechnology [CONACyT, 402901 to C.G.M.]
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Mycobacterium tuberculosis ; Increased sensitivity ; Sputum smear microscopy ; TB detection ; Nanotechnology ; Infectious disease
Publicat a:	Biosensors, Vol. 8 (december 2018) , ISSN 2079-6374

DOI: 10.3390/bios8040128
PMID: 30545099

13 p, 1.5 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats