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Extrachloroplastic PP7L functions in chloroplast development and abiotic stress tolerance
Xu, Duorong (Ludwig-Maximilians-Universität München)
Marino, Giada (Ludwig-Maximilians-Universität München)
Klingl, Andreas (Ludwig-Maximilians-Universität München)
Enderle, Beatrix (University of Freiburg)
Monte Collado, Elena (Centre de Recerca en Agrigenòmica)
Kurth, Joachim (Ludwig-Maximilians-Universität München)
Hiltbrunner, Andreas (University of Freiburg)
Leister, Dario (Ludwig-Maximilians-Universität München)
Kleine, Tatjana (Ludwig-Maximilians-Universität München)

Data: 2019
Resum: Chloroplast biogenesis is indispensable for proper plant development and environmental acclimation. In a screen for mutants affected in photosynthesis, we identified the protein phosphatase7-like (pp7l) mutant, which displayed delayed chloroplast development in cotyledons and young leaves. PP7L, PP7, and PP7-long constitute a subfamily of phosphoprotein phosphatases. PP7 is thought to transduce a blue-light signal perceived by crys and phy a that induces expression of SIGMA FACTOR5 (SIG5). We observed that, like PP7, PP7L was predominantly localized to the nucleus in Arabidopsis (Arabidopsis thaliana), and the pp7l phenotype was similar to that of the sig6 mutant. However, SIG6 expression was unaltered in pp7l mutants. Instead, loss of PP7L compromised translation and ribosomal RNA (rRNA) maturation in chloroplasts, pointing to a distinct mechanism influencing chloroplast development. Promoters of genes deregulated in pp7l-1 were enriched in PHYTOCHROME-INTERACTING FACTOR (PIF)-binding motifs and the transcriptome of pp7l-1 resembled those of pif and CONSTITUTIVE PHOTOMORPHOGENESIS1 (COP1) signalosome complex (csn) mutants. However, pif and csn mutants, as well as cop1, cryptochromes (cry)1 cry2, and phytochromes (phy)A phyB mutants, do not share the pp7l photosynthesis phenotype. PhyB protein levels were elevated in pp7l mutants, but phyB overexpression plants did not resemble pp7l. These results indicate that PP7L operates through a different pathway and that the control of greening and photosystem biogenesis can be separated. The lack of PP7L increased susceptibility to salt and high-light stress, whereas PP7L overexpression conferred resistance to highlight stress. Strikingly, PP7L was specifically recruited to Brassicales for the regulation of chloroplast development. This study adds another player involved in chloroplast biogenesis.
Drets: Tots els drets reservats
Llengua: Anglès.
Document: article ; recerca ; publishedVersion
Publicat a: Plant physiology, Vol. 180, Issue 1 (May 2019) , p. 323-341, ISSN 1532-2548

DOI: 10.1104/pp.19.00070
PMID: 30760637


19 p, 2.5 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > CRAG (Centre de Recerca en Agrigenòmica)
Articles > Articles de recerca
Articles > Articles publicats

 Registre creat el 2019-07-18, darrera modificació el 2019-08-12



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