Web of Science: 2 citations, Scopus: 2 citations, Google Scholar: citations,
Low-Cost Strategy for the Development of a Rapid Electrochemical Assay for Bacteria Detection Based on AuAg Nanoshells
Russo, Lorenzo (Institut Català de Nanociència i Nanotecnologia)
Leva Bueno, Juan (Institut Català de Nanociència i Nanotecnologia)
Bergua, José Francisco (Institut Català de Nanociència i Nanotecnologia)
Costantini, Monica (University of Parma)
Giannetto, Marco (University of Parma)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
De la Escosura Muñiz, Alfredo (Institut Català de Nanociència i Nanotecnologia)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date: 2018
Abstract: A low-cost strategy for the simple and rapid detection of bacterial cells in biological matrixes is presented herein. Escherichia coli and Salmonella typhimurium were chosen as model bacteria for the development of an electrochemical assay based on hollow AuAg nanoshells (NSs). By taking advantage of their electrocatalytic properties for the in situ generation of the electrochemical signal without the need of any other kind of reagent, substrate, or redox enzyme, high sensitivities (down to 10 CFU/mL) were achieved. Moreover, the recognition and discrimination of the model bacterial cells in the sample matrix was possible by relying solely on nonspecific affinity interactions between their cell walls and AuAg NSs surface, avoiding the use of expensive and fragile biological receptor. Compared to traditional, laboratory-based analytical tests available, this assay provides a promising proof-of-concept alternative that allows to obtain good sensitivities and selectivity in very short times in addition to the low cost.
Note: Número d'acord de subvenció MINECO/MAT2015-70725-R
Note: Número d'acord de subvenció AGAUR/2017/SGR-143
Note: Número d'acord de subvenció EC/H2020/685817
Note: Número d'acord de subvenció MINECO/SEV-2013-0295
Note: This is an open access article published under an ACS AuthorChoice License. See Standard ACS AuthorChoice/Editors' Choice Usage Agreement - https://pubs.acs.org/page/policy/authorchoice_termsofuse.html
Rights: Tots els drets reservats.
Language: Anglès.
Document: article ; recerca ; publishedVersion
Published in: ACS omega, Vol. 3, Issue 12 (December 2018) , p. 18849-18856, ISSN 2470-1343

DOI: 10.1021/acsomega.8b02458


8 p, 4.7 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles

 Record created 2019-07-26, last modified 2019-08-22



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