Low-Cost Strategy for the Development of a Rapid Electrochemical Assay for Bacteria Detection Based on AuAg Nanoshells
Russo, Lorenzo (Institut Català de Nanociència i Nanotecnologia)
Leva Bueno, Juan (Institut Català de Nanociència i Nanotecnologia)
Bergua Canudo, José Francisco (Institut Català de Nanociència i Nanotecnologia)
Costantini, Monica (University of Parma)
Giannetto, Marco (University of Parma)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
De La Escosura-Muñiz, Alfredo (Institut Català de Nanociència i Nanotecnologia)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Data:	2018
Resum:	A low-cost strategy for the simple and rapid detection of bacterial cells in biological matrixes is presented herein. Escherichia coli and Salmonella typhimurium were chosen as model bacteria for the development of an electrochemical assay based on hollow AuAg nanoshells (NSs). By taking advantage of their electrocatalytic properties for the in situ generation of the electrochemical signal without the need of any other kind of reagent, substrate, or redox enzyme, high sensitivities (down to 10 CFU/mL) were achieved. Moreover, the recognition and discrimination of the model bacterial cells in the sample matrix was possible by relying solely on nonspecific affinity interactions between their cell walls and AuAg NSs surface, avoiding the use of expensive and fragile biological receptor. Compared to traditional, laboratory-based analytical tests available, this assay provides a promising proof-of-concept alternative that allows to obtain good sensitivities and selectivity in very short times in addition to the low cost.
Ajuts:	Ministerio de Economía y Competitividad MAT2015-70725-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-143 European Commission 685817 Ministerio de Economía y Competitividad SEV-2013-0295
Nota:	This is an open access article published under an ACS AuthorChoice License. See Standard ACS AuthorChoice/Editors' Choice Usage Agreement - https://pubs.acs.org/page/policy/authorchoice_termsofuse.html
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	ACS omega, Vol. 3, Issue 12 (December 2018) , p. 18849-18856, ISSN 2470-1343

DOI: 10.1021/acsomega.8b02458

8 p, 4.7 MB

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