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Pàgina inicial > Articles > Articles publicats > Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy |
Data: | 2016 |
Resum: | In the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i. e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells. |
Ajuts: | European Commission 290023 Ministerio de Economía y Competitividad SEV-2013-0295 |
Drets: | Tots els drets reservats. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió acceptada per publicar |
Matèria: | Anti-cancer therapies ; Epidermal growth factor receptors ; Exohedral functionalization ; Functionalizations ; Functionalized carbon nanotubes ; High temperature treatments ; Secondary antibodies ; Single-walled carbon nanotube (SWCNTs) ; Animals ; Cell Line, Tumor ; Cetuximab ; CHO Cells ; Cricetulus ; Humans ; Metals ; Nanotubes, Carbon ; Neoplasms ; Radiotherapy ; Receptor, Epidermal Growth Factor |
Publicat a: | Nanoscale, Vol. 8, issue 25 (2016) , p. 12626-12638, ISSN 2040-3372 |
Postprint 19 p, 868.7 KB |