Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy
Spinato, Cinzia (Centre national de la recherche scientifique (França). Institut de Biologie Moléculaire et Cellulaire)
Pérez Ruiz De Garibay, Aritz (Centre national de la recherche scientifique (França). Institut de Biologie Moléculaire et Cellulaire)
Kierkowicz, Magdalena (Institut de Ciència de Materials de Barcelona)
Pach, Elzbieta (Institut Català de Nanociència i Nanotecnologia)
Martincic, Markus (Institut de Ciència de Materials de Barcelona)
Klippstein, Rebecca (King's College London. Institute of Pharmaceutical Science)
Bourgognon, Maxime (King's College London. Institute of Pharmaceutical Science)
Wang, Julie Tzu-Wen (King's College London. Institute of Pharmaceutical Science)
Ménard-Moyon, Cécilia (Centre national de la recherche scientifique (França). Institut de Biologie Moléculaire et Cellulaire)
Al-Jamal, Khuloud T. (King's College London. Institute of Pharmaceutical Science)
Ballesteros, Belén (Institut Català de Nanociència i Nanotecnologia)
Tobias, Gerard (Institut de Ciència de Materials de Barcelona)
Bianco, Alberto (Centre national de la recherche scientifique (França). Institut de Biologie Moléculaire et Cellulaire)

Data:	2016
Resum:	In the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i. e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells.
Ajuts:	European Commission 290023 Ministerio de Economía y Competitividad SEV-2013-0295
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Anti-cancer therapies ; Epidermal growth factor receptors ; Exohedral functionalization ; Functionalizations ; Functionalized carbon nanotubes ; High temperature treatments ; Secondary antibodies ; Single-walled carbon nanotube (SWCNTs) ; Animals ; Cell Line, Tumor ; Cetuximab ; CHO Cells ; Cricetulus ; Humans ; Metals ; Nanotubes, Carbon ; Neoplasms ; Radiotherapy ; Receptor, Epidermal Growth Factor
Publicat a:	Nanoscale, Vol. 8, issue 25 (2016) , p. 12626-12638, ISSN 2040-3372

DOI: 10.1039/c5nr07923c

Postprint 19 p, 868.7 KB

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