Label-free nanoplasmonic sensing of tumor-associate autoantibodies for early diagnosis of colorectal cancer
Soler Aznar, Maria (Institut Català de Nanociència i Nanotecnologia)
Estévez, M. Carmen (Institut Català de Nanociència i Nanotecnologia)
Villar-Vazquez, R. (Consejo Superior de Investigaciones Científicas (Espanya). Centro de Investigaciones Biológicas. Grupo de Proteómica Funcional)
Casal, José Ignacio (Consejo Superior de Investigaciones Científicas (Espanya). Centro de Investigaciones Biológicas. Grupo de Proteómica Funciona)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Date:	2016
Abstract:	Colorectal cancer is treatable and curable when detected at early stages. However there is a lack of less invasive and more specific screening and diagnosis methods which would facilitate its prompt identification. Blood circulating autoantibodies which are immediately produced by the immune system at tumor appearance have become valuable biomarkers for preclinical diagnosis of cancer. In this work, we present the rapid and label-free detection of colorectal cancer autoantibodies directly in blood serum or plasma using a recently developed nanoplasmonic biosensor. Our nanoplasmonic device offers sensitive and real-time quantification of autoantibodies with excellent selectivity and reproducibility, achieving limits of detection around 1 nM (150-160 ng mL⁻¹). A preliminary evaluation of clinical samples of colorectal cancer patients has shown good correlation with ELISA. These results demonstrate the reliability of the nanobiosensor strategy and pave the way towards the achievement of a sensitive diagnostic tool for early detection of colorectal cancer.
Grants:	Ministerio de Economía y Competitividad RTC-2014-1518-1 Ministerio de Economía y Competitividad SEV-2013-0295 Ministerio de Economía y Competitividad BIO2012-31023 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-624
Note:	M. Soler acknowledges financial support from " Programa de Formación de Personal Investigador (FPI) " from the Spanish Ministry of Economy and Competitiveness (MINECO). R. Villar-Vazquez was a recipient of a FPU fellowship from the Spanish Ministry of Education and Culture.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Nanoplasmonic biosensor ; Plasma ; Serum ; Colorectal cancer ; Autoantibodies ; Clinical diagnosis
Published in:	Analytica chimica acta, Vol. 930 (August 2016) , p. 31-38, ISSN 1873-4324

DOI: 10.1016/j.aca.2016.04.059

Postprint 58 p, 845.7 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles