Web of Science: 21 citations, Scopus: 21 citations, Google Scholar: citations,
Label-free bimodal waveguide immunosensor for rapid diagnosis of bacterial infections in cirrhotic patients
Maldonado, Jesús (Institut Català de Nanociència i Nanotecnologia)
González-Guerrero, Ana Belén (Institut Català de Nanociència i Nanotecnologia)
Domínguez Horna, Carlos (Institut de Microelectrònica de Barcelona)
Lechuga, Laura M. (Institut Català de Nanociència i Nanotecnologia)

Date: 2016
Abstract: Spontaneous bacterial peritonitis is an acute bacterial infection of ascitic fluid; it has a high incidence in cirrhotic patients and it is associated with high mortality. In such a situation, early diagnosis and treatment is crucial for the survival of the patient. However, bacterial analysis in ascitic fluid is currently based on culture methods, which are time-consuming and laborious. We report here the application of a photonic interferometer biosensor based on a bimodal waveguide (BiMW) for the rapid and label-free detection of bacteria directly in ascitic fluid. The device consists of a straight waveguide in which two modes of the same polarization interfere while interacting with the external medium through their evanescent fields. A bimolecular event occurring on the sensor area of the device (e. g. capturing bacteria) will differently affect each light mode, inducing a variation in the phase of the light exiting at the output of the waveguide. In this work, we demonstrate the quantitative detection of Bacillus cereus in buffer medium and Escherichia coli in undiluted ascitic fluid from cirrhotic patients. In the case of Bacillus cereus detection, the device was able to specifically detect bacteria at relevant concentrations in 12. 5 min and in the case of Escherichia coli detection, the analysis time was 25 min. Extrapolation of the data demonstrated that the detection limits of the biosensor could reach few bacteria per milliliter. Based on the results obtained, we consider that the BiMW biosensor is positioned as a promising new clinical tool for user-friendly, cost-effective and real-time microbiological analysis.
Note: Número d'acord de subvenció MICINN/TEC2012-34280
Note: Número d'acord de subvenció AGAUR/2014/SGR-624
Note: Número d'acord de subvenció MINECO/SEV-2013-0295
Rights: Tots els drets reservats.
Language: Anglès.
Document: article ; recerca ; submittedVersion
Subject: Bimodal waveguide biosensor ; Label-free immunoassay ; Bacillus Cereus ; Escherichia coli ; Ascitic fluid
Published in: Biosensors and bioelectronics, Vol. 85 (November 2016) , p. 310-316, ISSN 1873-4235

DOI: 10.1016/j.bios.2016.04.095


Preprint
20 p, 835.0 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles

 Record created 2019-09-23, last modified 2020-02-08



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