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Pàgina inicial > Articles > Articles publicats > Ketoisophorone synthesis with an immobilized alcohol dehydrogenase |
Data: | 2019 |
Resum: | The monoterpenoid α-isophorone is sourced from the available and renewable plant dry matter, as well as a waste recovery operation from acetone. This compound, can be hydroxylated to 4-hydroxy-isophorone which is the main precursor for the synthesis of ketoisophorone. On its turn, ketoisophorone is a key intermediate for the production of carotenoids and Vitamin E. Here, the enzymatic oxidation of 4-hydroxy-isophorone to ketoisophorone is demonstrated employing an alcohol dehydrogenase (ADHaa) from Artemisia annua and a NADPH oxidase (NOX), as a cofactor regeneration enzyme. After 24 h of reaction and an initial substrate concentration of 50 mM, 95. 7 % yield and a space time yield of 6. 52 g L⁻¹ day⁻¹ could be obtained. Furthermore, the immobilization of the alcohol dehydrogenase was studied on 17 different supports. An epoxy-functionalized agarose resulted in the highest metrics, 100±0% immobilization yield and 58. 2±3. 5 % retained activity. Finally, the immobilized ADHaa was successfully implemented in 4 reaction cycles (96 h operation) presenting a biocatalyst yield of 23. 4 g product g⁻¹ of enzyme. It represents a 2. 5-fold increase compared with the reaction with soluble enzymes. |
Ajuts: | European Commission 635734 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1462 |
Nota: | Altres ajuts: Authors also thank COST Action CM 1303-Systems Biocatalysis for financial support. |
Drets: | Tots els drets reservats. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió acceptada per publicar |
Matèria: | Alcohol dehydrogenase ; Biocatalysis ; Immobilization ; Ketoisophorone ; Recycling |
Publicat a: | CHEMCACHEM, Vol. 11, issue 19 (Oct. 2019) , p. 4862-4870, ISSN 1867-3880 |
Post-print 20 p, 1.1 MB |