Beyond the scavenging of reactive oxygen species (Ros) : direct effect of cerium oxide nanoparticles in reducing fatty acids content in an in vitro model of hepatocellular steatosis
Parra-Robert, Marina (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Casals, Eudald (Wuyi University. School of Biotechnology and Health Sciences)
Massana, Núria (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Zeng, Muling (Wuyi University. School of Biotechnology and Health Sciences)
Perramón, Meritxell (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Fernández-Varo, Guillermo (Universitat de Barcelona. Departament de Biomedicina)
Morales Ruiz, Manuel (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
Jiménez, Wladimiro (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Casals, Gregori (Institut d'Investigacions Biomèdiques August Pi i Sunyer)

Date:	2019
Abstract:	Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic accumulation of lipids. Antisteatotic effects of cerium oxide nanoparticles (CeO₂NPs) have recently been shown in animal models of liver disease. However, it is unclear whether the activity of CeONPs is related solely to the decrease in oxidative stress or, in addition, they directly decrease liver fatty acid accumulation. To address this question, in this work, we used an in vitro model of hepatocellular steatosis, exposing HepG2 cells to oleic and palmitic acid. Cell uptake of CeO₂NPs and their effect on oxidative stress and viability of hepatic cells cultured with H₂O₂ were also evaluated. Results show that CeO₂NPs were uptaken by HepG2 cells and reduced oxidative stress and improved cell viability. Treatment with oleic and palmitic acid increased lipogenesis and the content of different fatty acids. CeO₂NPs reduced palmitic and stearic acid and most fatty acids consisting of more than 18 carbon atoms. These effects were associated with significant changes in elongase and desaturase activity. In conclusion, CeO₂NPs directly protected HepG2 cells from cell injury in oxidative stress conditions and reduced fatty acid content in steatotic conditions by inducing specific changes in fatty acid metabolism, thus showing potential in the treatment of NAFLD.
Grants:	Ministerio de Economía y Competitividad PI15-00077 Ministerio de Economía y Competitividad SAF2016-75358-R
Note:	Altres ajuts: Secretaria d'Universitats i Recerca del Departament d'Empresa i Coneixement de la Generalitat de Catalunya, convocatòria d'Indústria del Coneixement modalitat B", grant number 2018_PROD_00187 toW.J, cofinançat per la Unió Europea a través de la European Regional Development Fund (ERDF)
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Nonalcoholic fatty liver disease ; Steatosis ; Liver ; Cerium oxide nanoparticles ; Oxidative stress
Published in:	Biomolecules, Vol. 9 , issue 9 (Sep. 2019) , art. 425, ISSN 2218-273X

DOI: 10.3390/biom9090425
PMID: 31470518

16 p, 3.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles