Computational and experimental analysis identifies Arabidopsis genes specifically expressed during early seed development
Becerra Baeza, Cristian Marcelo del C. (Institut de Recerca i Tecnologia Agroalimentàries)
Puigdomènech, Pere, 1948- (Institut de Recerca i Tecnologia Agroalimentàries)
Vicient Sánchez, Carlos M. (Institut de Recerca i Tecnologia Agroalimentàries)

Date:	2006
Abstract:	Background: Plant seeds are complex organs in which maternal tissues, embryo and endosperm, follow distinct but coordinated developmental programs. Some morphogenetic and metabolic processes are exclusively associated with seed development. The goal of this study was to explore the feasibility of incorporating the available online bioinformatics databases to discover Arabidopsis genes specifically expressed in certain organs, in our case immature seeds. Results: A total of 11,032 EST sequences obtained from isolated immature seeds were used as the initial dataset (178 of them newly described here). A pilot study was performed using EST virtual subtraction followed by microarray data analysis, using the Genevestigator tool. These techniques led to the identification of 49 immature seed-specific genes. The findings were validated by RT-PCR analysis and in situ hybridization. Conclusion: We conclude that the combined in silico data analysis is an effective data mining strategy for the identification of tissue-specific gene expression.
Grants:	Ministerio de Ciencia y Tecnología BIO2001-1721 Ministerio de Ciencia y Tecnología BIO2004-01577
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	BMC genomics, Vol. 7 (February 2006) , art. 38, ISSN 1471-2164

DOI: 10.1186/1471-2164-7-38
PMID: 16504176

11 p, 1.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles