Two-photon neuronal and astrocytic stimulation with azobenzene-based photoswitches
Izquierdo-Serra, Mercè (Institut de Bioenginyeria de Catalunya)
Gascón Moya, Marta (Universitat Autònoma de Barcelona. Departament de Química)
Hirtz, Jan J. (Columbia University. Department of Biological Sciences)
Pittolo, Silvia (Institut de Bioenginyeria de Catalunya)
Poskanzer, Kira E. (Columbia University. Department of Biological Sciences)
Ferrer Mallofré, Èric (Universitat Autònoma de Barcelona. Departament de Química)
Alibes, Ramón (Universitat Autònoma de Barcelona. Departament de Química)
Busqué Sánchez, Félix (Universitat Autònoma de Barcelona. Departament de Química)
Yuste, Rafael (Columbia University. Department of Biological Sciences)
Hernando Campos, Jordi (Universitat Autònoma de Barcelona. Departament de Química)
Gorostiza, Pau (Institut de Bioenginyeria de Catalunya)

Date:	2014
Abstract:	Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision and tissue penetration of two-photon stimulation have never been investigated in these molecules. Here we demonstrate two-photon excitation of azobenzene-based protein switches and versatile strategies to enhance their photochemical responses. This enables new applications to control the activation of neurons and astrocytes with cellular and subcellular resolution.
Grants:	European Commission 210355 European Commission 335011 European Commission 270483 Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-277 Agència de Gestió d'Ajuts Universitaris i de Recerca 2012/FI_B01122 Ministerio de Ciencia e Innovación CTQ2008-06160 Ministerio de Economía y Competitividad CTQ2012-30853 Ministerio de Ciencia e Innovación CTQ2010-15380
Note:	This is an open access article published under an ACS AuthorChoice License. See Standard ACS AuthorChoice/Editors' Choice Usage Agreement - https://pubs.acs.org/page/policy/authorchoice_termsofuse.html
Rights:	Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Biochemical functions ; New applications ; Photochemical response ; Photochromic compound ; Protein switches ; Subcellular resolution ; Tissue penetrations ; Two-photon excitations ; Azo Compounds ; Cells, Cultured ; HEK293 Cells ; Humans ; Molecular Structure ; Photochemical Processes ; Proteins ; Protons
Published in:	Journal of the American Chemical Society, Vol. 136, Issue 24 (June 2014) , p. 8693-8701, ISSN 1520-5126

DOI: 10.1021/ja5026326
PMID: 24857186

9 p, 2.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Synthesis of Bioactive Organic Compounds and Functional Materials (SynOrgFUN)
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Group in Electrochemistry, Photochemistry and Organic Reactivity (GEFRO)
Articles > Research articles
Articles > Published articles