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Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development
Pajoro, Alice (Wageningen University. Laboratory of Molecular Biology)
Madrigal, Pedro (Polish Academy of Sciences. Institute of Plant Genetics)
Muiño, Jose M. (Max-Planck Institute for Molecular Genetics. Department of Computational Molecular Biology)
Matus, José Tomás (Centre de Recerca en Agrigenòmica)
Jin, Jian (Centre de Recerca en Agrigenòmica)
Mecchia, Martin A. (Instituto de Biología Molecular y Celular de Rosario (Argentina))
Debernardi, Juan M. (Instituto de Biología Molecular y Celular de Rosario (Argentina))
Palatnik, Javier F. (Instituto de Biología Molecular y Celular de Rosario (Argentina))
Balazadeh, Salma (University of Potsdam. Institute of Biochemistry and Biology)
Arif, Muhammad (University of Potsdam. Institute of Biochemistry and Biology)
Ó'Maoiléidigh, Diarmuid S. (Trinity College Dublin. Smurfit Institute of Genetics)
Wellmer, Frank (Trinity College Dublin. Smurfit Institute of Genetics)
Krajewski, Pawel (Polish Academy of Sciences. Institute of Plant Genetics)
Riechmann, José Luis (Institució Catalana de Recerca i Estudis Avançats)
Angenent, Gerco C. (Business Unit Bioscience. Plant Research International)
Kaufmann, Kerstin (University of Potsdam. Institute of Biochemistry and Biology)

Date: 2014
Abstract: Background: Development of eukaryotic organisms is controlled by transcription factors that trigger specific and global changes in gene expression programs. In plants, MADS-domain transcription factors act as master regulators of developmental switches and organ specification. However, the mechanisms by which these factors dynamically regulate the expression of their target genes at different developmental stages are still poorly understood. Results: We characterized the relationship of chromatin accessibility, gene expression, and DNA binding of two MADS-domain proteins at different stages of Arabidopsis flower development. Dynamic changes in APETALA1 and SEPALLATA3 DNA binding correlated with changes in gene expression, and many of the target genes could be associated with the developmental stage in which they are transcriptionally controlled. We also observe dynamic changes in chromatin accessibility during flower development. Remarkably, DNA binding of APETALA1 and SEPALLATA3 is largely independent of the accessibility status of their binding regions and it can precede increases in DNA accessibility. These results suggest that APETALA1 and SEPALLATA3 may modulate chromatin accessibility, thereby facilitating access of other transcriptional regulators to their target genes. Conclusions: Our findings indicate that different homeotic factors regulate partly overlapping, yet also distinctive sets of target genes in a partly stage-specific fashion. By combining the information from DNA-binding and gene expression data, we are able to propose models of stage-specific regulatory interactions, thereby addressing dynamics of regulatory networks throughout flower development. Furthermore, MADS-domain TFs may regulate gene expression by alternative strategies, one of which is modulation of chromatin accessibility.
Note: Número d'acord de subvenció EC/FP7/237909
Note: Número d'acord de subvenció MICINN/BFU2011-22734
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: article ; recerca ; publishedVersion
Subject: Arabidopsis ; Arabidopsis Proteins ; Chromatin ; Chromatin Assembly and Disassembly ; Flowers ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Homeodomain Proteins ; MADS Domain Proteins ; Protein Binding ; Transcription Factors
Published in: Genome biology, Vol. 15 (March 2014) , p. R41, ISSN 1474-760X

DOI: 10.1186/gb-2014-15-3-r41
PMID: 24581456

19 p, 3.0 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2020-01-31, last modified 2020-11-07

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