Home > Articles > Published articles > Label-free Bacteria Quantification in Blood Plasma by a Bioprinted Microarray Based Interferometric Point-of-Care Device |
Date: | 2019 |
Abstract: | Existing clinical methods for bacteria detection lack speed, sensitivity, and, importantly, point-of-care (PoC) applicability. Thus, finding ways to push the sensitivity of clinical PoC biosensing technologies is crucial. Here we report a portable PoC device based on lens-free interferometric microscopy (LIM). The device employs high performance nanoplasmonics and custom bioprinted microarrays and is capable of direct label-free bacteria (E. coli) quantification. With only one-step sample handling we offer a sample-to-data turnaround time of 40 min. Our technology features detection sensitivity of a single bacterial cell both in buffer and in diluted blood plasma and is intrinsically limited by the number of cells present in the detection volume. When employed in a hospital setting, the device has enabled accurate categorization of sepsis patients (infectious SIRS) from control groups (healthy individuals and noninfectious SIRS patients) without false positives/negatives. User-friendly on-site bacterial clinical diagnosis can thus become a reality. |
Grants: | European Commission 644956 Ministerio de Economía y Competitividad SEV-2013-0295 Ministerio de Economía y Competitividad FICTS-1420-27 Ministerio de Economía y Competitividad SEV-2015-0522 Ministerio de Economía y Competitividad TEC2016-75080-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1634 |
Rights: | Tots els drets reservats. |
Language: | Anglès |
Document: | Article ; recerca ; Versió sotmesa a revisió |
Subject: | Bacteria ; Sepsis ; Microarray ; Label-free detection ; Nanoplasmonics ; Plasma samples |
Published in: | ACS Sensors, Vol. 4, Issue 1 (January 2019) , p. 52-60, ISSN 2379-3694 |
Preprint 24 p, 1.4 MB |