QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map
Pereira, Lara (Centre de Recerca en Agrigenòmica)
Ruggieri, Valentino (Centre de Recerca en Agrigenòmica)
Pérez, S. (Centre de Recerca en Agrigenòmica)
Alexiou, Konstantinos G. (Centre de Recerca en Agrigenòmica)
Fernández, Marta (Semillas Fitó, S.L)
Jahrmann, Torben (Semillas Fitó, S.L.)
Pujol Abajo, Marta (Centre de Recerca en Agrigenòmica)
Garcia-Mas, Jordi (Centre de Recerca en Agrigenòmica)

Date:	2018
Abstract:	Background: Melon shows a broad diversity in fruit morphology and quality, which is still underexploited in breeding programs. The knowledge of the genetic basis of fruit quality traits is important for identifying new alleles that may be introduced in elite material by highly efficient molecular breeding tools. - Results: In order to identify QTLs controlling fruit quality, a recombinant inbred line population was developed using two commercial cultivars as parental lines: "Védrantais", from the cantalupensis group, and "Piel de Sapo", from the inodorus group. Both have desirable quality traits for the market, but their fruits differ in traits such as rind and flesh color, sugar content, ripening behavior, size and shape. We used a genotyping-by-sequencing strategy to construct a dense genetic map, which included around five thousand variants distributed in 824 bins. The RIL population was phenotyped for quality and morphology traits, and we mapped 33 stable QTLs involved in sugar and carotenoid content, fruit and seed morphology and major loci controlling external color of immature fruit and mottled rind. The median confidence interval of the QTLs was 942kb, suggesting that the high density of the genetic map helped in increasing the mapping resolution. Some of these intervals contained less than a hundred annotated genes, and an integrative strategy combining gene expression and resequencing data enabled identification of candidate genes for some of these traits. - Conclusion: Several QTLs controlling fruit quality traits in melon were identified and delimited to narrow genomic intervals, using a RIL population and a GBS-based genetic map.
Grants:	European Commission 6655919 Ministerio de Economía y Competitividad AGL2015-64625-C2-1-R Ministerio de Economía y Competitividad SEV-2015-0533
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Quantitative trait loci ; Melon ; Fruit quality ; Fruit morphology ; Genotyping-by-sequencing ; Genetic map
Published in:	BMC plant biology, Vol. 18 (2018) , art. 324, ISSN 1471-2229

DOI: 10.1186/s12870-018-1537-5
PMID: 30509167

17 p, 3.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
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