A quick and efficient hydroponic potato infection method for evaluating potato resistance and Ralstonia solanacearum virulence
Wang, Huijuan (Northwest A & F University. State Key Laboratory of Crop Stress Biology for Arid Areas)
Hu, Jinxue (Northwest A & F University. State Key Laboratory of Crop Stress Biology for Arid Areas)
Lu, Yao (Northwest A & F University. State Key Laboratory of Crop Stress Biology for Arid Areas)
Zhang, Mancang (Northwest A & F University. State Key Laboratory of Crop Stress Biology for Arid Areas)
Qin, Ning (Northwest A & F University. State Key Laboratory of Crop Stress Biology for Arid Areas)
Zhang, Ruize (Northwest A & F University. State Key Laboratory of Crop Stress Biology for Arid Areas)
He, Yizhe (Northwest A & F University. State Key Laboratory of Crop Stress Biology for Arid Areas)
Wang, Dongdong (Northwest A & F University. State Key Laboratory of Crop Stress Biology for Arid Areas)
Chen, Yue (Northwest A & F University. State Key Laboratory of Crop Stress Biology for Arid Areas)
Zhao, Cuizhu (Northwest A & F University. State Key Laboratory of Crop Stress Biology for Arid Areas)
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)
Valls, Marc (Centre de Recerca en Agrigenòmica)
Chen, Qin (Northwest A & F University. State Key Laboratory of Crop Stress Biology for Arid Areas)
Lu, Haibin (Northwest A & F University. State Key Laboratory of Crop Stress Biology for Arid Areas)

Data:	2019
Resum:	Background: Potato, the third most important crop worldwide, plays a critical role in human food security. Brown rot, one of the most destructive potato diseases caused by Ralstonia solanacearum, results in huge economic losses every year. A quick, stable, low cost and high throughout method is required to meet the demands of identification of germplasm resistance to bacterial wilt in potato breeding programs. Results: Here we present a novel R. solanacearum hydroponic infection assay on potato plants grown in vitro. Through testing wilt symptom appearance and bacterial colonization in aerial part of plants, we found that the optimum conditions for in vitro potato infection were using an OD 0. 01 bacterial solution suspended with tap water for infection, broken potato roots and an open container. Infection using R. solanacearum strains with differential degree of aggressivity demonstrated that this infection system is equally efficient as soil-drench inoculation for assessment of R. solanacearum virulence on potato. A small-scale assessment of 32 potato germplasms identified three varieties highly resistant to the pathogen, which indicates this infection system is a useful method for high-throughout screening of potato germplasm for resistance. Furthermore, we demonstrate the utility of a strain carrying luminescence to easily quantify bacterial colonization and the detection of latent infections in hydroponic conditions, which can be efficiently used in potato breeding programs. Conclusions: We have established a quick and efficient in vitro potato infection system, which may facilitate breeding for new potato cultivars with high resistance to R. solanacearum.
Ajuts:	Ministerio de Economía y Competitividad AGL2016-78002-R Ministerio de Economía y Competitividad SEV-2015-0533
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Ralstonia solanacearum ; Potato ; In vitro infection ; Brown rot ; Bacterial wilt
Publicat a:	Plant methods, Vol. 15 (November 2019) , art. 145, ISSN 1746-4811

DOI: 10.1186/s13007-019-0530-9
PMID: 31798671

11 p, 3.5 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > CRAG (Centre de Recerca en Agrigenòmica)
Articles > Articles de recerca
Articles > Articles publicats