A model based on the combination of ifn-γ, ip-10, ferritin and 25-hydroxyvitamin d for discriminating latent from active tuberculosis in children
Comella-del-Barrio, Patricia (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Abellana, Rosa (Universitat de Barcelona. Facultat de Medicina i Ciències de la Salut)
Villar-Hernández, Raquel (Institut Germans Trias i Pujol)
Coute, M. D. J. (Saint-Damien Pediatric Hospital)
Mingels, B. S. (Radiology and Imaging Diagnose Department. Manso Primary Care Center)
Canales-Aliaga, Lydia (Hospital Universitari MútuaTerrassa (Terrassa, Catalunya))
Narcisse, M. (Saint-Damien Pediatric Hospital)
Gautier, Jacqueline (Saint-Damien Pediatric Hospital)
Ascaso, C. (Universitat de Barcelona. Facultat de Medicina i Ciències de la Salut)
Latorre, Irene (Institut Germans Trias i Pujol)
Domínguez, J.. (Institut Germans Trias i Pujol)
Pérez-Porcuna, Tomás M. (Universitat de Barcelona. Hospital Mútua de Terrassa)
Universitat Autònoma de Barcelona

Date:	2019
Abstract:	In recent years, pediatric research on tuberculosis (TB) has focused on addressing new biomarkers with the potential to be used as immunological non-sputum-based methods for the diagnosis of TB in children. The aim of this study was to characterize a set of cytokines and a series of individual factors (ferritin, 25-hydroxyvitamin D [25(OH)D], parasite infections, and nutritional status) to assess different patterns for discriminating between active TB and latent TB infection (LTBI) in children. The levels of 13 cytokines in QuantiFERON-TB Gold In-Tube (QFT-GIT) supernatants were analyzed in 166 children: 74 with active TB, 37 with LTBI, and 55 uninfected controls. All cytokines were quantified using Luminex or ELISA. Ferritin and 25(OH)D were also evaluated using CLIA, and Toxocara canis Ig-G antibodies were detected with a commercial ELISA kit. The combination of IP-10, IFN-γ, ferritin, and 25(OH)D achieved the best diagnostic performance to discriminate between active TB and LTBI cases in children in relation to the area under receiver operating characteristic (ROC) curve 0. 955 (confidence interval 95%: 0. 91-1. 00), achieving optimal sensitivity and specificity for the development of a new test (93. 2 and 90. 0%, respectively). Children with TB showed higher ferritin levels and an inverse correlation between 25(OH)D and IFN-γ levels. The model proposed includes a combination of biomarkers for discriminating between active TB and LTBI in children to improve the accuracy of TB diagnosis in children. This combination of biomarkers might have potential for identifying the onset of primary TB in children.
Grants:	Instituto de Salud Carlos III PI-16-1912
Note:	Altres ajuts: This work was supported by grants from: (i) the Isolana Foundation, (ii) the Maria Francisca de Roviralta Foundation expedient AT/MA 1-19/07/2017, AT/MA 70-27/04/2016, and AT/MA 3-22/10/2014 and (vi) Fundació Recerca i Docència Mútua Terrassa.
Note:	Altres ajuts: PERIS/SLT006-17-144
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Mycobacterium tuberculosis ; Biomarkers ; Cytokines ; Enzyme-linked immunoassays ; Ferritin ; Immune response ; Pediatrics ; Vitamin D
Published in:	Frontiers in microbiology, Vol. 10 Núm. AUG (2019) , p. 1855, ISSN 1664-302X

DOI: 10.3389/fmicb.2019.01855
PMID: 31474956

15 p, 638.1 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles