Home > Articles > Published articles > Calorie restriction prevents diet-induced insulin resistance independently of PGC-1-driven mitochondrial biogenesis in white adipose tissue |
Date: | 2019 |
Abstract: | Calorie restriction (CR) exerts remarkable, beneficial effects on glucose homeostasis bymechanisms that are not fully understood. Given the relevance of white adipose tissue (WAT) in glucose homeostasis, we aimed at identifying the main cellular processes regulated inWAT in response to CR in a pathologic context of obesity. For this, a gene-expression profiling studywas first conducted inmice fed ad libitum or subjected to 40%CR. We found that the genenetwork relatedtomitochondriawas themosthighlyupregulatedinWATbyCR. To study the role that increased mitochondrial biogenesis plays on glucose homeostasis following CR, we generated a mouse model devoid of the coactivators peroxisome proliferator-activated receptor γ coactivator 1 (PGC-1)a and PGC-1b specificallyinadipocytes. Our results showthatmice lackingPGC-1s inadipocytes areunable to increase mitochondrial biogenesis in WAT upon CR. Despite a blunted induction of mitochondrial biogenesis in response to calorie deprivation, mice lacking adipose PGC-1s still respond to CR by improving their glucose homeostasis. Our study demonstrates that PGC-1 coactivators are major regulators of CR-induced mitochondrial biogenesis in WAT and that increased mitochondrial biogenesis and oxidative function in adipose tissue are not required for the improvement of glucose homeostasismediated byCR. |
Grants: | Ministerio de Economía y Competitividad SAF2012-39484 Ministerio de Economía y Competitividad SAF2011-23886 Instituto de Salud Carlos III PI15-00701 |
Rights: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Language: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Subject: | Oxidative metabolism ; Adipocytes ; Glucose homeostasis ; Mitochondria |
Published in: | FASEB Journal, Vol. 33 Núm. 2 (2019) , p. 2343-2358, ISSN 1530-6860 |
16 p, 2.2 MB |