Web of Science: 5 cites, Scopus: 5 cites, Google Scholar: cites
Fluid supplementation accelerates epithelial repair during chemical colitis
Burgueño, Juan F. (University of Miami-Leonard. Division of Gastroenterology.)
Lang, Jessica K. (Harvard T.H. Chan School of Public Health (Boston, Estats Units d'Amèrica))
Santander, Ana M. (University of Miami-Leonard. Division of Gastroenterology)
Fernández, Irina (University of Miami-Leonard. Division of Gastroenterology)
Fernandez, Ester (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Zaias, Julia (University of Miami-Leonard. Department of Pathology and Laboratory Medicine)
Abreu, Maria T. (University of Miami-Leonard. Division of Gastroenterology)

Data: 2019
Resum: The dextran sulfate sodium (DSS) model of colitis is a common animal model of inflammatory bowel disease that causes pain and distress. In this study, we aimed to determine whether fluid supplementation can be used as a welfare-based intervention to minimize animal suffering. C57Bl/6 females undergoing acute colitis by administration of 3% DSS in drinking water were supplemented with 1 mL intraperitoneal injections of NaCl and compared to non-supplemented control mice. Mouse behavior and locomotive activity were assessed on days 5-6 after DSS initiation by means of tail suspension, novel object recognition and open field activity tests. Mice were euthanized after either the acute (day 7) or the recovery phase (day 12) of colitis and inflammation, epithelial proliferation, and differentiation were assessed by means of histology, immunohistochemistry, quantitative PCR, and western blot. We found that fluid-supplemented mice had reduced signs of colitis with no alterations in behavior or locomotive activity. Furthermore, we observed an accelerated epithelial repair response after fluid hydration during the acute phase of colitis, characterized by increased crypt proliferation, activation of ERK1/2, and modulation of TGF-β1 expression. Consistent with these findings, fluid-supplemented mice had increased numbers of goblet cells, upregulated expression of differentiation markers for absorptive enterocytes, and reduced inflammation during the recovery phase. Our results show that fluid hydration does not reduce stress in DSS-treated mice but alters colitis evolution by reducing clinical signs and accelerating epithelial repair. These results argue against the routine use of fluid supplementation in DSS-treated mice.
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Matèria: Animals ; Colitis ; Dextran Sulfate ; Disease Models, Animal ; Female ; Fluid Therapy ; Injections, Intraperitoneal ; Intestinal Mucosa ; Mice, Inbred C57BL ; Motor Activity ; Saline Solution ; Wound Healing
Publicat a: PloS one, Vol. 14, Issue 4 (April 2019) , art. e0215387, ISSN 1932-6203

DOI: 10.1371/journal.pone.0215387
PMID: 31002683


20 p, 2.2 MB

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