Fish red blood cells modulate immune genes in response to bacterial inclusion bodies made of TNFα and a g-VHSV fragment
Puente Marin, Sara (Universidad Miguel Hernández de Elche. Departamento de Bioquímica y Biología Molecular)
Thwaite, Rosemary (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Mercado, Luis (Pontificia Universidad Católica de Valparaíso. Instituto de Biología)
Coll, Julio (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya))
Roher Armentia, Nerea (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Del Mar Ortega-Villaizan, Maria (Universidad Miguel Hernández de Elche. Departamento de Bioquímica y Biología Molecular)

Date:	2019
Abstract:	Fish Red-Blood Cells (RBCs) are nucleated cells that can modulate the expression of different sets of genes in response to stimuli, playing an active role in the homeostasis of the fish immune system. Nowadays, vaccination is one of the main ways to control and prevent viral diseases in aquaculture and the development of novel vaccination approaches is a focal point in fish vaccinology. One of the strategies that has recently emerged is the use of nanostructured recombinant proteins. Nanostructured cytokines have already been shown to immunostimulate and protect fish against bacterial infections. To explore the role of RBCs in the immune response to two nanostructured recombinant proteins, TNFα and a G-VHSV protein fragment, we performed different in vitro and in vivo studies. We show for the first time that rainbow trout RBCs are able to endocytose nanostructured TNFα and G-VHSV protein fragment in vitro, despite not being phagocytic cells, and in response to nanostructured TNFα and G-VHSV fragment, the expression of different immune genes could be modulated.
Grants:	Ministerio de Economía y Competitividad AGL2015-65129-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-345
Note:	Altres ajuts: this work was supported by the European Research Council fund to MO-V (ERC Starting Grant GA639249)
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	TNFα ; VHSV glycoprotein G ; Bacterial inclusion bodies ; Erythrocytes ; Immune response ; Red blood cells
Published in:	Frontiers in immunology, Vol. 10 (May 2019) , art. 1055, ISSN 1664-3224

DOI: 10.3389/fimmu.2019.01055
PMID: 31178858

11 p, 3.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles