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CENP-A binding domains and recombination patterns in horse spermatocytes
Cappelletti, Eleonora (Università degli Studi di Pavia. Dipartimento di Biologia e Biotecnologie "L. Spallanzani")
Piras, Francesca M. (Università degli Studi di Pavia. Dipartimento di Biologia e Biotecnologie "L. Spallanzani")
Badiale, Claudia (Università degli Studi di Pavia. Dipartimento di Biologia e Biotecnologie "L. Spallanzani")
Bambi, Marina (Università degli Studi di Pavia. Dipartimento di Biologia e Biotecnologie "L. Spallanzani")
Santagostino, Marco (Università degli Studi di Pavia. Dipartimento di Biologia e Biotecnologie "L. Spallanzani")
Vara, Covadonga (Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Masterson, Teri A. (National University of Ireland. Centre for Chromosome Biology)
Sullivan, Kevin F. (National University of Ireland. Centre for Chromosome Biology)
Nergadze, Solomon G. (Università degli Studi di Pavia. Dipartimento di Biologia e Biotecnologie "L. Spallanzani")
Ruiz-Herrera Moreno, Aurora (Universitat Autònoma de Barcelona. Departament de Biologia Cellular, Fisiologia i Immunologia)
Giulotto, Elena (Università degli Studi di Pavia. Dipartimento di Biologia e Biotecnologie "L. Spallanzani")

Date: 2019
Abstract: Centromeres exert an inhibitory effect on meiotic recombination, but the possible contribution of satellite DNA to this "centromere effect" is under debate. In the horse, satellite DNA is present at all centromeres with the exception of the one from chromosome 11. This organization of centromeres allowed us to investigate the role of satellite DNA on recombination suppression in horse spermatocytes at the stage of pachytene. To this aim we analysed the distribution of the MLH1 protein, marker of recombination foci, relative to CENP-A, marker of centromeric function. We demonstrated that the satellite-less centromere of chromosome 11 causes crossover suppression, similarly to satellite-based centromeres. These results suggest that the centromere effect does not depend on satellite DNA. During this analysis, we observed a peculiar phenomenon: while, as expected, the centromere of the majority of meiotic bivalent chromosomes was labelled with a single immunofluorescence centromeric signal, double-spotted or extended signals were also detected. Their number varied from 0 to 7 in different cells. This observation can be explained by positional variation of the centromeric domain on the two homologs and/or misalignment of pericentromeric satellite DNA arrays during homolog pairing confirming the great plasticity of equine centromeres.
Note: Número d'acord de subvenció MINECO/CGL2017-83802-P
Note: Número d'acord de subvenció MINECO/CGL2014-54317-P
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: article ; recerca ; publishedVersion
Subject: Centromeres ; DNA recombination ; Meiosis
Published in: Scientific reports, Vol. 9, issue 1 (Jan. 2019) , art. 15800, ISSN 2045-2322

DOI: 10.1038/s41598-019-52153-1
PMID: 31676881


10 p, 3.0 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles

 Record created 2020-06-03, last modified 2020-08-22



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