Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa
Cantero, Jon (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Grau Expósito, Judith (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Serra-Peinado, Carla (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Rosero, Daniela A. (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Luque-Ballesteros, Laura (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Astorga-Gamaza, Antonio (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Castellvi, Josep (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Sanhueza, Tamara (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Tapia, Gustavo (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Lloveras, Belen (Parc de Salut MAR de Barcelona)
Fernández, Marco A. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Prado, Julia G.. (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Sole-Sedeno, Josep-Maria (Institut Hospital del Mar d'Investigacions Mèdiques)
Tarrats, Antoni (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Lecumberri, Carla (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Mañalich Barrachina, Laura (Hospital Universitari Vall d'Hebron)
Centeno Mediavilla, María Cristina (Hospital Universitari Vall d'Hebron)
Falcó, Vicenç (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Buzón, Maria José (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Genescà Ferrer, Meritxell (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Universitat Autònoma de Barcelona

Date:	2019
Abstract:	HIV viral reservoirs are established very early during infection. Resident memory T cells (T) are present in tissues such as the lower female genital tract, but the contribution of this subset of cells to the pathogenesis and persistence of HIV remains unclear. Here, we show that cervical CD4T display a unique repertoire of clusters of differentiation, with enrichment of several molecules associated with HIV infection susceptibility, longevity and self-renewing capacities. These protein profiles are enriched in a fraction of CD4T expressing CD32. Cervical explant models show that CD4T preferentially support HIV infection and harbor more viral DNA and protein than non-T. Importantly, cervical tissue from ART-suppressed HIV women contain high levels of viral DNA and RNA, being the T fraction the principal contributor. These results recognize the lower female genital tract as an HIV sanctuary and identify CD4T as primary targets of HIV infection and viral persistence. Thus, strategies towards an HIV cure will need to consider T phenotypes, which are widely distributed in tissues.
Grants:	Ministerio de Ciencia e Innovación R21AI118411 Ministerio de Ciencia e Innovación SAF2015-67334-R Instituto de Salud Carlos III PI14-01058 Instituto de Salud Carlos III PI17-00164 Instituto de Salud Carlos III RD16-0025-0007 Instituto de Salud Carlos III CP17-00179 Instituto de Salud Carlos III CP15-00014
Note:	Altres ajuts: PERIS/SLT002-16-00353
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	HIV infections ; Viral reservoirs
Published in:	Nature communications, Vol. 10 Núm. 1 (january 2019) , p. 4739, ISSN 2041-1723

DOI: 10.1038/s41467-019-12732-2
PMID: 31628331

16 p, 2.5 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles