Total serum IgD from healthy and sick dogs with leishmaniosis
Martínez-Orellana, P. (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Maristany, C. (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Baxarias, Marta (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Álvarez-Fernández, A. (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Baldassarre, Antonella (Università Degli Studi di Bari Aldo Moro)
Ordeix, L. (Universitat Autònoma de Barcelona. Hospital Clínic Veterinari.)
Solano Gallego, Laia. (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Date:	2019
Abstract:	Background: Canine leishmaniosis (CanL) due to Leishmania infantum is characterized by the development of both cellular and humoral immune responses. The dysfunction of T cell-mediated immunity leads to a lack of proliferation of T cells in response to Leishmania antigens with the consequence of parasite dissemination that seems to be related to a T cell exhaustion mediated by regulatory B cells expressing immunoglobulin D (IgD). The aim of this study was to determine and compare the total serum IgD in dogs with clinical leishmaniosis and in clinically healthy dogs. Results: A total of 147 dog sera were studied. All dogs were tested for L. infantum-specific antibodies by quantitative ELISA. Interferon-gamma (IFN-γ) production was also determined by sandwich ELISA after blood stimulation with L. infantum soluble antigen (LSA) or concanavalin A (ConA). The quantification of total IgD was performed using a human IgD sandwich ELISA quantification set. Dogs were classified in three different groups. Group 1 included 40 clinically healthy non-infected dogs, all serologically negative to L. infantum-specific antibodies and non-producers of IFN-γ upon LSA stimulation. Group 2 included 63 clinically healthy infected dogs that were LSA IFN-γ producers (n = 61) and/or IFN-γ non-producers (n = 2) as well as negative to medium seropositive to L. infantum antigen. Finally, Group 3 included 44 dogs with clinical leishmaniosis (IFN-γ producers, n = 23; and IFN-γ non-producers, n = 21) that were negative to highly positive to L. infantum-specific antibodies. No significant differences were observed when the total IgD concentration was compared within groups. Additionally, total IgD of sick IFN-γ producers and IFN-γ non-producers was not significantly different. Finally, total IgD concentration was not statistically related to demographic parameters such as age, sex and breed. Conclusions: The results of this study demonstrated that there were no differences between groups in total serum IgD. Total serum IgD does not appear to be a marker of disease in CanL.
Grants:	Ministerio de Economía y Competitividad AGL2012‑32498
Note:	This study was supported by Spanish ministry grants, Ministerio de Economía y competitividad and Fondo Europeo de Desarrollo Regional (FEDER, EU) (AGL2012‑32498 and AGL2015‑68477).
Note:	Altres ajuts: FEDER/AGL2015‑68477
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Leishmania infantum ; Canine leishmaniosis ; IFN‑γ ; IL‑10 ; IgD ; PD‑1
Published in:	Parasites & vectors, Vol. 12 Núm. 1 (26 2019) , p. 119, ISSN 1756-3305

DOI: 10.1186/s13071-019-3384-0
PMID: 30909975

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
Articles > Research articles
Articles > Published articles