Web of Science: 0 citas, Scopus: 0 citas, Google Scholar: citas
Matrix effect in the isolation of breast cancer- derived nanovesicles by immunomagnetic separation and electrochemical immunosensing- A comparative study
Moura, Silio Lima (Universitat Autònoma de Barcelona. Departament de Química. Grup de Sensors i Biosensors)
Martí Ripoll, Mercè (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Pividori, María Isabel (Universitat Autònoma de Barcelona. Departament de Química. Grup de Sensors i Biosensors)
Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí"

Fecha: 2020
Resumen: Exosomes are cell-derived nanovesicles released into biological fluids, which are involved in cell-to-cell communication. The analysis of the content and the surface of the exosomes allow conclusions about the cells they are originating from and the underlying condition, pathology or disease. Therefore, the exosomes are currently considered good candidates as biomarkers to improve the current methods for clinical diagnosis, including cancer. However, due to their low concentration, conventional procedures for exosome detection including biosensing usually require relatively large sample volumes and involve preliminary purification and preconcentration steps by ultracentrifugation. In this paper, the immunomagnetic separation is presented as an alternative method for the specific isolation of exosomes in serum. To achieve that, a rational study of the surface proteins in exosomes, which can be recognized by magnetic particles, is presented. The characterization was performed in exosomes obtained from cell culture supernatants of MCF7, MDA- MB-231 and SKBR3 breast cancer cell lines, including TEM and nanoparticle tracking analysis (NTA). For the specific characterization by flow cytometry and confocal microscopy, different commercial antibodies against selected receptors were used, including the general tetraspanins CD9, CD63 and CD81, and cancer-related receptors (CD24, CD44, CD54, CD326 and CD340). The effect of the serum matrix on the immunomagnetic separation was then carefully evaluated by spiking the exosomes in depleted human serum. Based on this study, the exosomes were preconcentrated by immunomagnetic separation on antiCD81-modified magnetic particles in order to achieve further magnetic actuation on the surface of the electrode for the electrochemical readout. The performance of this approach is discussed and compared with classical characterization methods.
Nota: Número d'acord de subvenció MINECO/BIO2016-75751-R
Derechos: This is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Creative Commons
Lengua: Anglès
Documento: article ; recerca ; publishedVersion
Publicado en: Sensors, Vol. 20, issue 4 (2020) , art. 965, ISSN 1424-8220

DOI: 10.3390/s20040965
PMID: 32054015

21 p, 2.9 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Instituto de Biotecnología y de Biomedicina (IBB)
Artículos > Artículos de investigación
Artículos > Artículos publicados

 Registro creado el 2020-06-22, última modificación el 2020-09-10

   Favorit i Compartir