mcr -Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain
Migura-Garcia, Lourdes (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
González-López, Juanjo 1975- (Hospital Universitari Vall d'Hebron)
Martinez-Urtaza, Jaime (Centre for Environment, Fisheries and Aquaculture Science)
Aguirre Sánchez, José Roberto (Instituto de Investigación en Recursos Cinegéticos)
Moreno-Mingorance, Albert (Hospital Universitari Vall d'Hebron)
Pérez de Rozas, Ana (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Höfle, Ursula (Instituto de Investigación en Recursos Cinegéticos)
Ramiro, Y. (Instituto de Investigación en Recursos Cinegéticos)
Gonzalez-Escalona, Narjol (Food and Drug Administration)

Data:	2020
Resum:	Colistin has become the last-line antimicrobial for the treatment of multidrug resistant (MDR) Enterobacterales in human medicine. To date, several colistin resistance genes have been described. Of them mcr -1 is disseminated worldwide in Escherichia coli of human and animal origin. The aim of this study was to characterize mcr -mediated resistance plasmids from E. coli of animal origin in Spain. From our strain collection, 70 E. coli of pig origin collected between 2005 and 2014 (10 per year, except for years 2009-2010-2013) were randomly selected and screened for the presence of mcr -genes. Additionally, 20 E. coli isolated in 2011 from white storks (Ciconia ciconia) from the same urban household waste landfill associated colony were also included. Whole genome sequencing of mcr -positive isolates was carried out on a MiSeq (Illumina). Hybrid whole genome sequencing strategy combining nanopore and Illumina technologies were performed in a selection of isolates to close the genomes and plasmids and identify the presence of antimicrobial resistance genes. Minimum inhibitory concentration (MIC) was used to assess the susceptibility to colistin. Mating experiments were carried out to evaluate transferability of the mcr -genes. A total of 19 mcr -1 and one mcr -4 positive isolates were detected, 15 from pigs distributed during the study period, and five from storks collected in 2011. No other mcr -variants were found. The MICs for colistin ranged between 4 and.
Ajuts:	Ministerio de Economía y Competitividad RTI2018-095586-B-C22
Nota:	Altres ajuts: MINSAN/FIS PI15/00604
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Escherichia coli ; Colistin ; Mcr -plasmids ; MinION nanopore ; Pigs ; Storks ; Spain
Publicat a:	Frontiers in microbiology, Vol. 10 (january 2020) , ISSN 1664-302X

DOI: 10.3389/fmicb.2019.03072
PMID: 32010114

11 p, 2.1 MB

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