Bartonella spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain
Portillo, Aránzazu (Hospital Universitario San Pedro-Centro de Investigación Biomédica de La Rioja. Departamento de Enfermedades Infecciosas)
Maggi, Ricardo (North Carolina State University. Comparative Medicine Institute)
Oteo, José A. (Hospital Universitario San Pedro-Centro de Investigación Biomédica de La Rioja. Departamento de Enfermedades Infecciosas)
Bradley, Julie (North Carolina State University. Comparative Medicine Institute)
García-Álvarez, Lara (Hospital Universitario San Pedro-Centro de Investigación Biomédica de La Rioja. Departamento de Enfermedades Infecciosas)
San-Martín, Montserrat (Universidad de Granada. Facultad de Ciencias Sociales y Juridicas)
Roura, Xavier (Universitat Autònoma de Barcelona. Hospital Clínic Veterinari)
Breitschwerdt, Edward (North Carolina State University. Comparative Medicine Institute)

Date:	2020
Abstract:	Bartonella spp. are increasingly implicated in association with a spectrum of zoonotic infectious diseases. One hundred sanitary workers in La Rioja, Spain, completed a questionnaire and provided blood specimens for Bartonella spp. serology and Bartonella Alpha-Proteobacteria growth medium (BAPGM) enrichment blood culture/PCR. Six immunofluorescence assays (IFA) were performed and aseptically obtained blood specimens were inoculated into liquid BAPGM and subcultured onto blood agar plates. Bartonella DNA was amplified using conventional and real-time PCR assays. The Bartonella spp. , strain, or genotype was determined by DNA sequencing. Bartonella seroreactivity was documented in 83. 1% and bloodstream infection in 21. 6% of participants. Bartonella henselae, B. vinsonii subsp. berkhoffii genotypes I and III, and B. quintana were identified. IFA seroreactivity and PCR positivity were not statistically associated with self-reported symptoms. Our results suggest that exposure to and non-clinical infection with Bartonella spp. may occur more often than previously suspected in the La Rioja region.
Note:	This research was funded by Bayer Animal Health GmbH (Leverkusen, Germany), grant number 2111116219.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Bartonella Alpha-Proteobacteria Growth Medium (BAPGM), B. henselae ; B. quintana ; B. vinsonii subsp. berkhoffii ; B. koehlerae ; Sanitary workers
Published in:	Pathogens, Vol. 9 (march 2020) , ISSN 2076-0817

DOI: 10.3390/pathogens9030189
PMID: 32143533

16 p, 319.5 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
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