Antimicrobial Activity and Prevention of Bacterial Biofilm Formation of Silver and Zinc Oxide Nanoparticle-Containing Polyester Surfaces at Various Concentrations for Use
Fontecha-Umaña, Fabio (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Ríos-Castillo, Abel Guillermo (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Ripolles-Avila, Carolina (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Rodríguez-Jerez, José Juan (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Date:	2020
Abstract:	Food contact surfaces are primary sources of bacterial contamination in food industry processes. With the objective of preventing bacterial adhesion and biofilm formation on surfaces, this study evaluated the antimicrobial activity of silver (Ag-NPs) and zinc oxide (ZnO-NPs) nanoparticle-containing polyester surfaces (concentration range from 400 ppm to 850 ppm) using two kinds of bacteria, Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli), and the prevention of bacterial biofilm formation using the pathogen Listeria monocytogenes. The results of antimicrobial efficacy (reductions ≥ 2 log CFU/cm 2) showed that at a concentration of 850 ppm, ZnO-NPs were effective against only E. coli (2. 07 log CFU/cm 2). However, a concentration of 400 ppm of Ag-NPs was effective against E. coli (4. 90 log CFU/cm 2) and S. aureus (3. 84 log CFU/cm 2). Furthermore, a combined concentration of 850 ppm Ag-NPs and 400 ppm ZnO-NPs showed high antimicrobial efficacy against E. coli (5. 80 log CFU/cm 2) and S. aureus (4. 11 log CFU/cm 2). The results also showed a high correlation between concentration levels and the bacterial activity of Ag-ZnO-NPs (R 2 = 0. 97 for S. aureus, and R 2 = 0. 99 for E. coli). They also showed that unlike individual action, the joint action of Ag-NPs and ZnO-NPs has high antimicrobial efficacy for both types of microorganisms. Moreover, Ag-NPs prevent the biofilm formation of L. monocytogenes in humid conditions of growth at concentrations of 500 ppm. Additional studies under different conditions are needed to test the durability of nanoparticle containing polyester surfaces with antimicrobial properties to optimize their use.
Grants:	Ministerio de Ciencia e Innovación RTI2018-098267-R-C32
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Nanoparticles ; Zinc oxide ; Silver ; Antimicrobial ; Biofilms ; Polyester ; Escherichia coli ; Staphylococcus aureus ; Listeria monocytogenes
Published in:	Foods, Vol. 9 (april 2020) , ISSN 2304-8158

DOI: 10.3390/foods9040442
PMID: 32268566

15 p, 953.8 KB

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