Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms
Vidal, Anna (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Aguirre Molins, Laia (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Seminati, Chiara (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Tello, Montse (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Redondo, Noelia (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Martín Castillo, Margarita (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)
Darwich Soliva, Laila (Institut de Recerca i Tecnologia Agroalimentàries. Centre de Recerca en Sanitat Animal)

Date:	2020
Abstract:	Escherichia coli is considered one of the most common agents associated with neonatal diarrhea in piglets. The aim of this work was to characterize the pathogenic and antimicrobial resistance (AMR) profiles of 122 E. coli strains isolated from pigs suffering diarrhea (n = 94) and pigs without diarrhea (n = 28) of 24 farms in Spain. Virulence factors, toxins and AMR (ESBL and colistin) genes and AMR phenotypes of E. coli isolates were analyzed. Low prevalence of pathogenic E. coli strains (26%) was found in both groups. However, ETEC and VTEC strains were more frequently isolated from diarrheic piglets. Irrespectively of diarrhea occurrence, 97. 5% of the strains showed a multidrug-resistance (MDR) profile to aminopenicillins, sulfonamides and tetracyclines. It was found that 22% of E. coli was CTX-M+, with CTX-M-14 being the principal allelic variant. Remarkably, 81. 5% of CTX-M+ strains were isolated from diarrheic animals and presented an extended MDR profile to aminopenicillins, quinolones and aminoglycosides. Finally, low frequencies of colistin resistance genes mcr -1 (4/122) and mcr -4 (1/122) were found. MDR E. coli strains are circulating in pig farms of Spain, representing a serious threat to animal and public health. More appropriate diagnostic approaches (genetic and AMR phenotypic analysis) should be implemented in animal health to optimize antibiotic treatments.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Antimicrobial resistance ; ESBL genes ; Colistin resistance ; Escherichia coli ; Neonatal diarrhea ; Pig
Published in:	Veterinary Sciences, Vol. 48, núm. 7 (april 2020) , ISSN 2306-7381

DOI: 10.3390/vetsci7020048
PMID: 32326282

12 p, 874.3 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles