Adaptation of hydroxymethylbutenyl diphosphate reductase enables volatile isoprenoid production
Bongers, Mareike (The University of Queensland. Australian Institute for Bioengineering and Nanotechnolog (Australia))
Pérez-Gil, Jordi (Centre de Recerca en Agrigenòmica)
Hodson, Mark P. (The University of Queensland. Australian Institute for Bioengineering and Nanotechnolog (Australia))
Schrübbers, Lars (Technical University of Denmark. Novo Nordisk Foundation Center for Biosustainability (Denmark))
Wulff, Tune (Technical University of Denmark. Novo Nordisk Foundation Center for Biosustainability (Denmark))
Sommer, Morten O. A. (Technical University of Denmark. Novo Nordisk Foundation Center for Biosustainability (Denmark))
Nielsen, Lars K. (The University of Queensland. Australian Institute for Bioengineering and Nanotechnolog (Australia))
Vickers, Claudia E. (CSIRO Synthetic Biology Future Science Platform (Australia))

Fecha:	2020
Resumen:	Volatile isoprenoids produced by plants are emitted in vast quantities into the atmosphere, with substantial effects on global carbon cycling. Yet, the molecular mechanisms regulating the balance between volatile and non-volatile isoprenoid production remain unknown. Isoprenoids are synthesised via sequential condensation of isopentenyl pyrophosphate (IPP) to dimethylallyl pyrophosphate (DMAPP), with volatile isoprenoids containing fewer isopentenyl subunits. The DMAPP:IPP ratio could affect the balance between volatile and non-volatile isoprenoids, but the plastidic DMAPP:IPP ratio is generally believed to be similar across different species. Here we demonstrate that the ratio of DMAPP:IPP produced by hydroxymethylbutenyl diphosphate reductase (HDR/IspH), the final step of the plastidic isoprenoid production pathway, is not fixed. Instead, this ratio varies greatly across HDRs from phylogenetically distinct plants, correlating with isoprenoid production patterns. Our findings suggest that adaptation of HDR plays a previously unrecognised role in determining in vivo carbon availability for isoprenoid emissions, directly shaping global biosphere-atmosphere interactions.
Ayudas:	European Commission 623679
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	eLife, Vol. 9 (March 2020) , art. e48685, ISSN 2050-084X

DOI: 10.7554/eLife.48685
PMID: 32163032

16 p, 1.5 MB

Figures and figure supplements 7 p, 2.4 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > CRAG (Centro de Investigación en Agrigenómica)
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