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Pàgina inicial > Articles > Articles publicats > RNA-binding and prion domains : |
Data: | 2020 |
Resum: | Proteins and RNAs assemble in membrane-less organelles that organize intracellular spaces and regulate biochemical reactions. The ability of proteins and RNAs to form condensates is encoded in their sequences, yet it is unknown which domains drive the phase separation (PS) process and what are their specific roles. Here, we systematically investigated the human and yeast proteomes to find regions promoting condensation. Using advanced computational methods to predict the PS propensity of proteins, we designed a set of experiments to investigate the contributions of Prion-Like Domains (PrLDs) and RNA-binding domains (RBDs). We found that one PrLD is sufficient to drive PS, whereas multiple RBDs are needed to modulate the dynamics of the assemblies. In the case of stress granule protein Pub1 we show that the PrLD promotes sequestration of protein partners and the RBD confers liquid-like behaviour to the condensate. Our work sheds light on the fine interplay between RBDs and PrLD to regulate formation of membrane-less organelles, opening up the avenue for their manipulation. |
Ajuts: | European Commission 309545 European Commission 855923 European Commission 311522 European Commission 825070 Ministerio de Economía y Competitividad BFU2014-55054-P Agencia Estatal de Investigación BFU2017-86970-P European Commission 727658 |
Nota: | Altres ajuts: CERCA Programme/Generalitat de Catalunya |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Publicat a: | Nucleic acids research, Vol. 48, Issue 17 (September 2020) , p. 9491-9504, ISSN 1362-4962 |
14 p, 3.1 MB |