Glutathione Ethyl Ester Protects In Vitro-Maturing Bovine Oocytes against Oxidative Stress Induced by Subsequent Vitrification/Warming
Garcia Martinez, Tania (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Ordóñez-León, Erika Alina (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Álvarez-Rodríguez, Manuel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
López Béjar, Manel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Yeste Oliveras, Marc (Universitat de Girona. Institut de Tecnologia Agroalimentària)
Mogas Amorós, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Vendrell-Flotats, Meritxell (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Martínez-Rodero, Iris (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Data:	2020
Resum:	This study aimed to examine whether the addition of glutathione ethyl ester (GSH-OEt) to the in vitro maturation (IVM) medium would improve the resilience of bovine oocytes to withstand vitrification. The effects of GSH-OEt on spindle morphology, levels of reactive oxygen species (ROS), mitochondrial activity and distribution, and embryo developmental potential were assessed together with the expression of genes with a role in apoptosis (BAX, BCL2), oxidative-stress pathways (GPX1, SOD1), water channels (AQP3), implantation (IFN-τ) and gap junctions (CX43) in oocytes and their derived blastocysts. Vitrification gave rise to abnormal spindle microtubule configurations and elevated ROS levels. Supplementation of IVM medium with GSH-OEt before vitrification preserved mitochondrial distribution pattern and diminished both cytoplasmic and mitochondrial ROS contents and percentages of embryos developing beyond the 8-cell stage were similar to those recorded in fresh non-vitrified oocytes. Although not significantly different from control vitrified oocytes, vitrified oocytes after GSH-OEt treatment gave rise to similar day 8-blastocyst and hatching rates to fresh non-vitrified oocytes. No effects of GSH-OEt supplementation were noted on the targeted gene expression of oocytes and derived blastocysts, with the exception of GPX1, AQP3 and CX43 in derived blastocysts. The addition of GSH-OEt to the IVM medium before vitrification may be beneficial for embryo development presumably as the consequence of additional anti-oxidant protection during IVM.
Ajuts:	Ministerio de Ciencia e Innovación AGL2016-79802-P Agència de Gestió d'Ajuts Universitaris i de Recerca 2017-SGR 1229
Nota:	Altres ajuts: GC/2017_FI_00451
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Oòcits -- Criopreservació ; Bestiar boví -- Cria i millora ; Cow ; Reactive oxygen species ; Cryopreservation ; Cryotop ; Spindle configuration ; Mitochondria ; Embryo development ; Gene expression
Publicat a:	International journal of molecular sciences, Vol. 21 Núm. 20 (2020) , p. 7547, ISSN 1422-0067

DOI: 10.3390/ijms21207547
PMID: 33066129

22 p, 2.7 MB

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