High-throughput cell motility studies on surface-bound protein nanoparticles with diverse structural and compositional characteristics
Tatkiewicz, Witold I. (Institut de Ciència de Materials de Barcelona)
Seras-Franzoso, Joaquin (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Garcia-Fruitos, Elena (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Vázquez Gómez, Esther (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Kyvik, Adriana R. (Institut de Ciència de Materials de Barcelona)
Ventosa, Nora (Institut de Ciència de Materials de Barcelona)
Guasch, Judith (Institut de Ciència de Materials de Barcelona)
Villaverde Corrales, Antonio (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Veciana i Miró, Jaume (Institut de Ciència de Materials de Barcelona)
Ratera Bastardas, Imma (Institut de Ciència de Materials de Barcelona)
Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia

Fecha:	2019
Resumen:	Eighty areas with different structural and compositional characteristics made of bacterial inclusion bodies formed by the fibroblast growth factor (FGF-IBs) were simultaneously patterned on a glass surface with an evaporation-assisted method that relies on the coffee-drop effect. The resulting surface patterned with these protein nanoparticles enabled to perform a high-throughput study of the motility of NIH-3T3 fibroblasts under different conditions including the gradient steepness, particle concentrations, and area widths of patterned FGF-IBs, using for the data analysis a methodology that includes "heat maps". From this analysis, we observed that gradients of concentrations of surface-bound FGF-IBs stimulate the total cell movement but do not affect the total net distances traveled by cells. Moreover, cells tend to move toward an optimal intermediate FGF-IB concentration (i. e. , cells seeded on areas with high IB concentrations moved toward areas with lower concentrations and vice versa, reaching the optimal concentration). Additionally, a higher motility was obtained when cells were deposited on narrow and highly concentrated areas with IBs. FGF-IBs can be therefore used to enhance and guide cell migration, confirming that the decoration of surfaces with such IB-like protein nanoparticles is a promising platform for regenerative medicine and tissue engineering.
Ayudas:	Ministerio de Economía y Competitividad MAT2016-80826-R Ministerio de Economía y Competitividad MAT2013-50036EXP Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-918 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-229 European Commission 654360 European Commission 600388 Agència de Gestió d'Ajuts Universitaris i de Recerca TECSPR15-1-0015 Ministerio de Economía y Competitividad SEV-2015-0496
Nota:	Altres ajuts: CERCA Programme/Generalitat de Catalunya i the COST Action CA15126. Post-Doctoral fellowship from Asociación Española Contra el Cáncer (AECC), post-doctoral fellowship from INIA (DOC-INIA) i ICREA Academia award
Derechos:	Tots els drets reservats.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Inclusion Bodies ; Cell motility ; High throughput ; Tissue engineering ; Concentration gradients ; Surface patterning ; Protein nanoparticles
Publicado en:	ACS biomaterials science & engineering, Vol. 5, Issue 10 (October 2019) , p. 5470-5480, ISSN 2373-9878

DOI: 10.1021/acsbiomaterials.9b01085

Postprint 32 p, 2.1 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Instituto de Biotecnología y de Biomedicina (IBB)
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