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Página principal > Artículos > Artículos publicados > Nanostructured recombinant protein particles raise specific antibodies against the nodavirus NNV coat protein in sole |
Fecha: | 2020 |
Resumen: | Nervous necrosis virus (NNV) reassortant strains RGNNV/SJNNV have emerged as a potent threat to the Mediterranean marine aquaculture industry, causing viral encephalopathy and retinopathy (VER) in Senegalese sole (Solea senegalensis). In this study, a cheap and practical vaccine strategy using bacterial inclusion bodies made of the coat protein of a virulent reassortant strain of this betanodavirus was devised. The nanostructured recombinant protein nanoparticles, VNNV-C, were administered without adjuvant to two groups of juvenile sole, one by intraperitoneal injection and the other by oral intubation. Specific antibodies were raised in vivo against the NNV coat protein via both routes, with a substantial specific antibody expansion in the injected group 30 days post homologous prime boost. Expression levels of five adaptive immune-related genes, cd8a, cd4, igm, igt and arg2, were also quantified in intestine, spleen and head kidney. Results showed cd4 and igm were upregulated in the head kidney of injected fish, indicating activation of an adaptive systemic response, while intubated fish exhibited a mucosal response in the intestine. Neither route showed significant differential expression of cd8a. The specific antibody response elicited in vivo and the lack of any signs of toxicity over the 6-week study period in young fish (n = 100), evidences the potential of the nanoparticle as a vaccine candidate. |
Ayudas: | Ministerio de Economía y Competitividad AGL2015-65129-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-345 Ministerio de Economía y Competitividad AGL2014-53190-REDC |
Nota: | Altres ajuts: Fondo Europeo Marítimo y de la Pesca 2014-2020 and IFAPA project PP.FEM.PPA201700.15 |
Derechos: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. |
Lengua: | Anglès |
Documento: | Article ; recerca ; Versió acceptada per publicar |
Materia: | NNV ; Protein nanoparticles ; Vaccine ; Oral gavage ; Antibodies ; Mucosal immunity ; Viral antigens |
Publicado en: | Fish and Shellfish Immunology, Vol. 99 (April 2020) , p. 578-586, ISSN 1095-9947 |
Article. Postprint 19 p, 527.1 KB |
Material acompanyament 6 p, 959.6 KB |
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